垂体肿瘤转化基因1在肝细胞癌患者中的表达及意义

Expression and significance of pituitary tumor-transforming gene 1 in patients with hepatocellular carcinoma

目的探讨垂体肿瘤转化基因(PTTG)1在肝细胞癌(肝癌)患者肝组织中的表达及意义。方法收集中山大学附属第三医院2011年7月至2016年12月确诊的60例肝癌肝切除病理组织标本和2016年6月至12月行肝癌肝切除的15例新鲜肝癌及癌旁组织标本。患者均签署知情同意书,符合医学伦理学规定。以同期因肝血管瘤行肝叶切除的远离血管瘤正常肝组织24例作为正常对照组。免疫组化法、Western blot、RT-PCR染色检测肝癌组织PTTG1表达。PTTG1表达与临床参数的相关性研究采用t检验、Mann-Whitney检验、Kruskal-Wallis检验。率的比较采用χ~2检验或Fisher确切概率法。结果免疫组化检测显示肝癌和癌旁组织的PTTG1阳性率分别为65%(5%,80%)、3%(2%,6%),明显高于正常对照组织的2%(1%,2%)(Z=6.644,3.876;P<0.05)。Western blot检测显示肝癌组织和癌旁组织的PTTG1相对表达量分别为0.88(0.80,1.78)、0.83(0.53,1.27),明显高于正常对照组织的0.36(0.20,0.62)(Z=3.223,2.605;P<0.05)。RT-PCR检测显示肝癌组织PTTG1相对mRNA表达量为3.09(1.46,4.77),明显高于癌旁组织的0.90(0.62,1.14)(Z=2.605,P<0.05)。肝癌组织PTTG1蛋白表达与组织分化程度有关(χ~2=3.849,P<0.05);而癌旁组织PTTG1表达与患者年龄、血清α-L-岩藻糖苷酶水平和肿瘤体积有关(t=2.463,χ~2=4.342,6.070;P<0.05)。结论PTTG1在肝癌组织中高表达,且PTTG1蛋白表达与肝癌患者α-L-岩藻糖苷酶水平、肿瘤大小、分化程度等相关。

Objective To investigate the expression and significance of pituitary tumor-transforming gene（PTTG）1 in the liver tissues of patients with hepatocellular carcinoma（HCC）.Methods 60 HCC pathological tissues of patients undergoing hepatectomy from July 2011 to December 2016 and 15 fresh HCC and para-cancerous tissues of patients undergoing hepatectomy from June to December 2016 in the Third Affiliated Hospital of Sun Yat-sen University were collected.The informed consents of all patients were obtained and the local ethical committee approval was received.Normal liver tissues distant from the hemangioma of 24 patients undergoing lobectomy were collected as normal controls.The expression level of PTTG1 in HCC tissues was detected by immunohistochemistry,Western blot and RT-PCR.The correlation between expression levels of PTTG1 and clinical parameters of patients were analyzed using t test,Mann-Whitney test and Kruskal-Wallis test.The rate comparison was conducted by Chi-square test or Fisher＇s exact test.Results Immunohistochemical staining demonstrated that the positive rate of PTTG1 in HCC tissues and para-cancerous tissues were 65%（5%,80%）and 3%（2%,6%）,significantly higher than 2%（1%,2%）in normal control tissues（Z=6.644,3.876;P0.05）.Western blot revealed that the relative expression levels of PTTG1 in HCC tissues and para-cancerous tissues were 0.88（0.80,1.78）and 0.83（0.53,1.27）,significantly higher than 0.36（0.20,0.62）in normal control tissues（Z=3.223,2.605;P0.05）.RT-PCR showed that the relative expression of PTTG1 mRNA in HCC tissues was 3.09（1.46,4.77）,significantly higher than 0.90（0.62,1.14）in para-cancerous tissues（Z=2.605,P0.05）.In HCC tissues,the expression of PTTG1 protein was correlated with the degree of differentiation（χ^2=3.849,P0.05）.The PTTG1 expression in para-cancerous tissues was correlated with age,serum level ofα-L-fucosidase and tumor size（t=2.463,χ^2=4.342,6.070;P0.05）.Conclusions PTTG1 is highly expressed in HCC tissues.The expression of PTTG1 protein is correlated with the serum level ofα-L-fucosidase,tumor size and degree of differentiation in HCC patients.