摘要
目的:探讨miR-519d在胰腺癌细胞中的表达及作用。方法:用qRT-PCR检测miR-519d在胰腺癌细胞系AsPC-1、BxPC-3、Capan-2、Panc-1及正常胰腺导管上皮细胞系HPDE6-C7中的表达。将Panc-1细胞分别转染miR-519d过表达质粒(miR-519d组)与阴性对照质粒(阴性对照组),以无处理的Panc-1细胞为空白对照组,用MTT法、Transwell实验、Western blot分别检测转染后细胞的增殖和侵袭能力、凋亡情况以及X连锁凋亡抑制蛋白(XIAP)的表达。结果:各胰腺癌细胞系中miR-519d相对表达量均明显低于正常胰腺导管上皮细胞系HPDE6-C7(均P<0.05);与空白对照组比较,miR-519d组细胞增殖能力降低(培养72 h后明显降低)、凋亡率明显升高、侵袭能力明显减弱、XIAP蛋白表达量明显降低(均P<0.05);阴性对照组各项指标与空白对照组差异均无统计学意义(均P>0.05)。结论:miR-519d在胰腺癌细胞中表达下调,miR-519d表达下调有增强胰腺癌细胞增殖和侵袭能力、降低凋亡的作用,该作用可能与其调节XIAP蛋白的表达有关。
Objective: To investigate the miR-519d expression in pancreatic cancer cells, and its actions. Methods: The miR-519d expressions in pancreatic cancer cell lines AsPC-1, BxPC-3, Capan-2 and Panc-1 as well as in normal pancreatic duct epithelial cell line HPDE6-C7 were detected by qRT-PCR. The Panc-1 cells were transfected with miR-519d over-expression plasmid (miR-519d group) and negative plasmid (negative control group) respectively, with untreated Panc-1 cells as blank control group. After that, the proliferative and invasion abilities, apoptosis and expression of X-linked inhibitor of apoptosis protein (XIAP) were determined by MTT assay, Transwell assay, flow cytometry and Western blot analysis, respectively. Results: The relative expression level of miR-519d in each pancreatic cancer cell line was significantly lower than that in normal pancreatic duct epithelial HPDE6-C7 cells (all P〈0.05). In cells of miR-519d group compared with blank control group, the proliferative ability was reduced (significantly reduced after 72-h culture), the apoptosis rate was significantly increased, the invasion ability was significantly decreased and the expression level of XIAP protein was significantly down-regulated (all P〈0.05). All the differences of the observed parameters in negative control group and blank control group showed no statistical significance (all P〉0.05). Conclusion: The miR-519d expression is down-regulated in pancreatic cancer cells. Down-regulation of miR- 519d expression can enhance the proliferative and invasion abilities, and decrease apoptosis in pancreatic cancer cell lines, and these actions may be associated with its regulating XIAP protein expression.
作者
梁治坤
程凡天
胡走肖
蔡常春
LIANG Zhikun, CHENG Fantian, HU Zouxiao, CAI Changchun(Department of Hepatopancreatobiliary Surgery, Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China)
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2018年第9期1142-1147,共6页
China Journal of General Surgery
关键词
胰腺肿瘤
微RNAS
细胞增殖
细胞凋亡
肿瘤侵润
Pancreatic Neoplasms
MicroRNAs
Cell Proliferation
Apoptosis
Neoplasm Invasiveness