精神分裂症患者PI3K-AKT基因表达水平与临床症状的关系

Relationship between PI3K-AKT gene expression levels and clinical symptoms in schizophrenia patients

目的探讨急慢性精神分裂症患者外周血淋巴细胞磷酸肌醇-3激酶-蛋白激酶B（PI3K-AKT）的mRNA表达水平及其与临床症状的关系。方法以qRT-PCR技术检测急性精神分裂症组（24例）、长期服药（氯氮平）慢性精神分裂症组（19例）和对照组（20例）外周血淋巴细胞中PI3K亚单位B（PI3KCB）、AKT1的mRNA表达水平，同时用PANSS量表对精神分裂症患者的阳性和阴性症状进行评定，并采用Spearman分析二者的相关性。结果急性精神分裂症组、长期服药慢性精神分裂症组、对照组样本外周血淋巴细胞中PI3KCB的基因表达水平分别为（0．79±0．04）、（0．83±0．08）、（0．87±0．09），三组比较差异有统计学意义（F=8．77，P=0．001）；AKT1的基因表达水平分别为（0．80±0．03）、（0．27±0．13）、（0．29±0．12），三组比较差异有统计学意义（F=302．31，P〈0．01）；急性精神分裂症患者的PI3KCB mRNA水平明显低于健康对照组（MD=0．09，P=0．002），急性精神分裂症患者的AKT1 mRNA水平显著高于慢性精神分裂症患者的水平（MD=0．53，P〈0．01）和健康对照者的水平（MD=0．51，P〈0．01），未在两组精神分裂症患者中发现PI3KCB、AKT1的表达水平与PANSS评分之间的相关性。结论PI3K-AKT途径的基因表达状态在急慢性精神分裂症患者有显著差异，但与临床症状无显著相关性，且氯氮平治疗可能影响其基因表达水平。

Objective To detect the mRNA expression levels of PI3KCB and AKT1 genes in the peripheral blood lymphocytes of acute and chronic schizophrenia patients in different stages, and to explore the relationship between them and the clinic symptoms. Methods Twenty-four cases of schizophrenia patients without medication for at least 1 month, 19 chronic schizophrenia patients with long-term clozapine medication,and 20 normal controls were involved in the study. The mRNA expression of PI3KCB and AKT1 genes of all the subjects were measured by real-time qRT-PCR, and the positive and negative symptom scales （PANSS） of schizophrenia patients were also evaluated. Spearman＇s correlation was used to analyze the relationship between PI3KCB, AKTI and PANSS score. Results The gene expression of PI3KCB in acute schizophrenia patients, chronic schizophrenia patients with clozapine medication and normal control were （0. 79±0. 04）, （0. 83±0.08） and （0. 87±0.09） respectively, and the difference was significant among the three groups （ F= 8.77,P= 0. 001 ）. The AKT1 gene expression levels were （ 0.80±0. 03） , （ 0. 27±0. 13 ） and （0. 29±0. 12） respectively, and the difference was also significant among the three groups （F= 302.31, P〈0.01 ）. The PI3KCB mRNA levels of acute schizophrenia patients were significantly lower than the levels in healthy controls （MD = 0. 09, P= 0. 002） , and the AKT1 mRNA levels of acute schizophrenia patients were significantly higher than the levels in chronic schizophrenia patients （ MD = 0. 53 ,P〈0.01 ） and healthy controis （MD = 0. 51, P〈0. 01 ）. In schizophrenia patients, no significant relationship was found between PI3KCB, AKT1 expression levels and PANSS scores. Conclusion The gene expression status of PI3K-AKT pathway is significantly different in different stages of acute and chronic schizophrenia and that is no significant relationship with clinic symptoms, and clozapine treatment may affect its gene expression levels.