猴真菌监测分析

Monitoring and analysis of monkey fungi

目的:了解猴真菌携带情况,为猴致病性真菌感染防控提供科学依据。方法:用真菌培养、Taq Man-MGB探针实时荧光定量PCR、普通PCR、基因克隆和测序技术,进行猴的真菌监测分析。对所有真菌分离株进行抗真菌药敏试验。结果:从全国几个不同厂家122只猴中分离获得71株真菌,形态和分子鉴定确证这些真菌为阿萨希毛孢子菌、圆形毛孢子菌、白色念珠菌、黄曲霉、烟曲霉、黑曲霉、焦曲霉、杂色曲霉、聚多曲霉、匿名曲霉、产黄青霉、草酸青霉、变幻青霉、宛氏拟青霉、小刺青霉、绳状青霉、卷枝毛霉、多变根毛霉、伞枝犁头霉、球毛壳霉、热带头梗霉、节菱孢霉菌、暗孢节菱孢菌、链格孢、松色二孢菌。结果表明,猴真菌种类比较丰富,获得真菌11属25种,毛孢子菌属、曲霉属、青霉属、毛霉属、念珠菌属真菌在这些致病真菌中占主导地位。抗真菌药敏试验分析结果显示:这些真菌分离株对制霉菌素、两性霉素B、氟康唑、咪康唑、酮康唑、氟胞嘧啶、特比萘芬已产生了耐药性。结论:联合使用真菌培养、Taq Man-MGB探针实时荧光定量PCR、普通PCR、基因克隆和测序技术能有效分析猴真菌。猴的真菌大多为人兽共患病病原体。研究结果为我国猴真菌监测提供参考数据,为致病性真菌的侵袭防治提供科学依据。

Objective： To understand the carrying condition of monkey fungus, to provide scientific basis for the prevention and control of pathogenic fungal infection in monkeys. Methods： The monkey fungi were monitored and analyzed by means of fungal culture, TaqMan-minor groove binder probe based real-time fluorescence quantitative polymerase chain reaction （ TaqMan-MGB probe RTFQ-PCR ）, conventional PCR, gene cloning and sequencing techniques. Antifungal susceptibility test was carried out for all fungi were isolated from 122 monkeys from several different manufacturers in identification confirmed these fungi as Trichosporon asahii, Trichosporon fungi isolates. Results： Seventy-one China. Morphological and molecular montevideense , Candida albicans ,Aspergillus flavus , Aspergillus fumigatus , Aspergillus niger, Aspergillus ustus , Aspergillus versicolor , Aspergillus sydowii, Aspergillus nomius , Penicillium chrysogenum, Penicillium oxalicum, Penicillium variabile , Paecilomyces variotii, Penicillium spinulosum , Penicillium funiculosum, Mucor circinelloides , Rhizomucor variabilis , Absidia corymbifera , Chaetomium globosum , Cephaliophora tropica , Arthrinium ovatum , Arthrinium phaeospermum , Alternaria alternata and Diplodia pinea. The results showed that the species of monkey fungi were richness and diverse. Twenty-five species of fungi which belong to 11 genera were obtained. The genus Trichosporon, Aspergillus, Penicillium, Mucor and Candida were dominant in these pathogenic fungi. The determination of the antifungal sensitivity test showed that these fungal strains were resistant to nystatin, amphotericin B, fluconazole, miconazole, ketoconazole, fluorocytosine and terbinafine. Conclusion： Combined use of fungal culture, TaqMan- MGB probe RTFQ-PCR, conventional PCR, gene cloning and sequencing technology can effectively analyze the monkey fungi. Most of monkey fungi were zoonotic pathogens. The results provide reference data for monkey monitoring in China and provide scientific basis for the prevention and treatment of pathogenic fungi. fungus