油樟标准提取物的指纹图谱研究

GC fingerprint of Cinnamomum longepaniculatum (Gamble) N.Chao standard extract

目的:建立油樟标准提取物GC指纹图谱分析方法,为其质量评价提供依据。方法:采用气相色谱法。采用石英毛细管HP-5柱(30.0 m×0.32 mm×0.25μm);氢火焰离子化检测器;进样口温度195℃;检测器温度230℃;程序升温:初始温度70℃,保持14 min,以1.5℃·min-1的速率升温至85℃,保持6 min。使用该方法对35批油樟标准提取物进行指纹图谱测定,并以共有峰的相对峰面积值为参数,对35批油樟标准提取物进行相似度评价和聚类分析。结果:35批油樟标准提取物的GC指纹图谱有11个共有峰,相似度评价和聚类分析结果表明,35批油樟标准提取物虽来自于6个厂家,提取设备和人员操作可能存在差异,但其成分的比例和含量接近,品质相近。结论:该方法分析时间短,对油樟标准提取物中各成分的分离效率高,其精密度、重复性和稳定性均符合指纹图谱研究的技术要求(RSD<3%),可作为油樟标准提取物质量评价的参考依据。

Objective：To establish a GC chromatographic fingerprint analysis method for the further quality evaluation of Cinnamomum longepaniculatum（Gamble）N.Chao standard extract.Methods：GC was adopted.The determination was performed on an HP-5 column（30.0 m×0.32 mm×0.25 μm.Hydrogen flame ionization detector was used with inlet temperature of 195 ℃.The temperature of the detector was 230 ℃.The controlled temperature programming was as follows：The initial temperature was 70 ℃ and maintained for 14 min,and then was raised to 85 ℃ at rate the of 1.5 ℃·min-1 for 6 min.The chromatographic fingerprints of 35 batches of Cinnamomum longepaniculatum （Gamble）N.Chao standard extract were determined.And the similarity and cluster analysis were carried out according to the relative peak area of characteristic peaks.Results：The GC chromatographic fingerprints of 35 batches of Cinnamomum longepaniculatum（Gamble）N.Chao standard extract showed 11 characteristic peaks,and the result of similarity evaluation and cluster analysis indicated that 35 batches of Cinnamomum longepaniculatum（Gamble）N.Chao standard extract from 6 manufacturers might differ in extraction equipment Cann,but the ratio and content of their ingredients as well as their quality were similar.onnd cpluerssioonnel operatio：The proposed method was fast and effective,whose precision,stability and reproducibility met the technical requirements of fingerprint（RSD3%）.It provided reference for quality evaluation of Cinnamomum longepaniculatum（Gamble）N.Chao standard extract.