摘要
A real-time label-free DNA biosensor based on thin-core fiber(TCF) interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers(SMFs) and integrated into a microfluidic channel. By modifying the TCF surface with monolayer poly-l-lysine(PLL) and single-stranded deoxyribonucleic acid(ssDNA) probes, the target DNA molecules can be captured in the microfluidic channel. The transmission spectra of the biosensor are measured and theoretically analyzed under different biosensing reaction processes. The results show that the wavelength has a blue-shift with the process of the DNA hybridization. Due to the advantages of low cost, simple operation as well as good detection effect on DNA molecules hybridization, the proposed biosensor has great application prospects in the fields of gene sequencing, medical diagnosis, cancer detection and environmental engineering.
A real-time label-free DNA biosensor based on thin-core fiber （TCF） interferometer is demonstrated experimentally. The proposed biosensor is constructed by splicing a TCF between two segments of single mode fibers （SMFs） and in- tegrated into a microfluidic channel. By modifying the TCF surface with monolayer poly-l-lysine （PLL） and sin- gle-stranded deoxyribonucleic acid （ssDNA） probes, the target DNA molecules can be captured in the microfluidic channel. The transmission spectra of the biosensor are measured and theoretically analyzed under different biosensing reaction processes. The results show that the wavelength has a blue-shift with the process of the DNA hybridization. Due to the advantages of low cost, simple operation as well as good detection effect on DNA molecules hybridization, the proposed biosensor has great application prospects in the fields of gene sequencing, medical diagnosis, cancer de- tection and environmental engineering.
作者
龙少聪
朱彦儒
胡慕芸
祁一凡
蒋韵睿
刘波
张旭
LONG Shao-cong;ZHU Yan-ru;HU Mu-yun;QI Yi-fan;JIANG Yun-rui;LIU Bo;and ZHANG Xu(Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology,Institute of Modern Optics,Nankai University,Tianjin 300350,China)
基金
supported by the National Undergraduate Innovation Training Program(No.201710055065)
