猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)最小免疫剂量的研究

Determination of the Minimum Immunical Dose of Live Vaccine aginst Swine Pseudorabies(Strain Bartha-K61,Cell Line Origin)

为了确定猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)的最小免疫剂量,本研究将3批猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)分别稀释成10TCID_(50)/mL、102.0TCID_(50)/mL、103.0TCID_(50)/mL,每批疫苗各个稀释度分别免疫仔猪1.0 mL/头,并设攻毒对照组和阴性对照组。免后10 d连同攻毒对照组用伪狂犬病病毒GD1株进行攻毒保护试验,阴性对照组不攻毒。结果表明,10TCID_(50)/头和102.0TCID_(50)/头的免疫剂量在免疫后10 d依然无法提供完全的免疫保护,保护率为20%～80%(1/5～4/5);103.0TCID_(50)/头的免疫剂量能够保护仔猪抵抗PRV强毒的攻击,保护率为100%(5/5);攻毒对照组发病率为100%(5/5),死亡率为80%(4/5);阴性对照组全部健活。由此确定猪伪狂犬病活疫苗(Bartha-K61株,传代细胞源)最小免疫剂量为103.0TCID_(50)/头。

In order to determine the minimum immunical dose of Swine Pseudorabies Live Vaccine（Strain Bartha-K61,Cell Line Origin）,Three batches of swine Pseudorabies live vaccines were diluted to 10TCID50,10^2.0TCID50, and 10^3.0TCID50 per dose, respectively. Each batch of vaccine was immunized with piglets at each dilution, and a challenged group and a unchallenged group were set up.After 10 days of immunizatio, the Pseudorabies virus GD1 strain was used for the challenge protection test, and the unchallenged group was not challenged. The results showed that 10TCID50 and 10^2.0TCID50 groups failed to provide complete protection, the protection rate was 20%-80%. The 10^3.0TCID50 groups could provide complete protection, the protection rate was 100%. All challenged piglets were diseased and 80% died. The unchallenged piglets were healthy. According to the above results, the minimal effective dose of vaccines against Pseudorabies virus was 10^3.0TCID50 pre piglet.