湘产土牛膝中蜕皮甾酮类化合物分离与鉴定

Study on the Ecdysterone Constituents of Achyranthes aspera L.

目的研究湘产特色药材土牛膝化学成分。方法采用乙醇提取、溶剂萃取法初步分离,硅胶、反相硅胶柱层析与溶剂重结晶进行化合物分离纯化,通过波谱数据、理化性质及文献对照对分离得到的化合物进行结构鉴定。结果从土牛膝乙醇提取物中获得7个蜕皮甾酮类化合物,分别为:β-蜕皮甾酮(1)、牛膝甾酮(2)、水龙骨甾酮B(3)、pterosterone(4)、24(28)-ecdysterone(5)、achyranthesterone A(6)、rubrosterone(7)。结论化合物4-7均为首次从该种植物中分离得到。

Objective To study the chemical constituents of Achyranthes aspera L. produced in Hunan. Methods The constituents were separated and purified by silica gel and ODS column chromatography and their structures were identified on the basis of physicochemical properties, spectral data and reference. Results Seven compounds were isolated and identified as β-ecdysterone（1）, inokosterone （2）, polypodine B （3）, pterosterone （4）, 24（28）-ecdysterone （5）, achyranthesterone A （6）, rubrosterone （7）. Conclusion Compounds 4-7 were isolated from this plant for the first time.