三七总皂苷对A549细胞侵袭转移的影响及机制研究

The research on the effect and mechanism of Panax notoginseng saponins on invasion and metastasis of A549 cells

目的探讨三七总皂苷(PNS)对A549细胞侵袭转移的影响及机制。方法根据培养方法的不同将A549细胞分为对照组、PNS低剂量组(15 mg/L)、PNS中剂量组(30 mg/L)和PNS高剂量组(60 mg/L)。采用Transwell细胞侵袭实验检测A549细胞侵袭转移能力,q RT-PCR及Western blot法检测A549细胞mi RNA-379-5p、基质金属蛋白酶(MMP)-2及MMP-9的表达情况。结果与对照组比较,PNS低、中、高剂量组中A549细胞侵袭转移能力均下降,mi RNA-379-5p表达上调,MMP-2的m RNA及蛋白表达下调,差异均有统计学意义(P<0.05或P<0.01);PNS中、高剂量组中MMP-9 m RNA及蛋白表达下调(P<0.05)。结论 PNS可抑制A549细胞侵袭转移,其机制可能与PNS上调A549细胞mi RNA-379-5p表达和下调MMP-2、MMP-9表达有关。

Objective To investigate the effect and mechanism of Panax notoginseng saponins （PNS） on invasion and metastasis of A549 cells. Methods A549 cells were divided into control group, low dose of PNS group （15 mg/L）, medi- um dose of PNS group （30 rag/L）, high dose of PNS group （60 rag/L） according to different culture methods. Transwell cell invasion test was used to detect the invasion and metastasis ability of A549 cells, qRT-PCR and Western blot methods were used to detect the expression levels of miRNA-379-5p, matrix metalloproteinase （MMP）-2 and MMP-9 in A549 cells. Results Compared with control group, the invasion and metastasis ability of A549 cells of low, medium, high dose of PNS groups decreased, the expression of miRNA-379-5p increased, the expression of MMP-2 protein and mRNA decreased, the differences were statistically significant （P 〈 0.05 or P 〈 0.01）; the expression of MMP-9 protein and mRNA of medium and high dose of PNS groups decreased （P 〈 0.05）. Conclusion PNS could inhibit the invasion and metastasis of A549 cells, and its mechanism may be related to upregulation of miRNA-379-5p expression and downregulation of MMP-2 and MMP-9 expression in A549 cells induced by PNS.