PRDX3在姜黄素减轻布比卡因诱导的SH-SY5Y细胞凋亡中的作用及其机制研究

Effect and mechanism of PRDX3 in attenuation of bupivacaine-induced cell apoptosis in SH-SY5Y cells by curcumin

目的研究PRDX3在姜黄素减轻布比卡因诱导的SH-SY5Y细胞凋亡中的作用及其机制。方法构建布比卡因诱导的人神经母细胞瘤细胞SH-SY5Y模型,与不同浓度姜黄素预孵育后加入布比卡因培养,采用MTT方法检测细胞活力,流式细胞仪方法检测细胞凋亡率,RT-PCR和Western blot测定抗氧化蛋白PRDX3 mRNA和蛋白表达量变化,并观察细胞形态学改变。结果布比卡因会引起SH-SY5Y细胞凋亡,姜黄素预处理可以显著降低布比卡因诱导的细胞毒性,减少凋亡率,抗氧化蛋白PRDX3的表达增加可能是姜黄素神经保护的机制之一。结论 PRDX3的高表达是姜黄素对于局麻药造成的神经损伤的保护作用的可能机制。

Objective To study the effects and mechanism of PRDX3 in attenuation of bupivacaine-induced cell apoptosis in SH-SY5Y cells by curcumin. Methods The human neuroblastoma cells induced by SH SY5Y model were builded, with bupivacaine culture after beforehand incubation by different concentrations of curcumin, MTT method was used to detect the cell vitality, flow cytometry instrument method was used to detect apoptosis rate, RT-PCR and Western blot was used to detect antioxidants PRDX3 mRNA and protein expression changes, and cell morphological changes were observed. Results Bupivacaine caused SH-SY5Y cells apoptosis, curcumin pretreatment can significantly reduce the cytotoxicity induced by bupivacaine, reduce the apoptosis rate, increased the expression of protein oxidation PRDX3, and maybe one of the curcumin neural protection mechanism. Conclusion The high expression of PRDX3 is a possible mechanism of the protective effects of curcumin on nerve damage caused by local anesthetics.