摘要
RNA聚合酶Ⅱ最大亚基Rpb1的羧基端结构域(carboxyl-terminal repeat domain,CTD)是RNA聚合酶Ⅱ发挥转录延伸功能所必需的,对其执行精确的转录调节功能至关重要。酵母细胞周期蛋白依赖性激酶CTDK-Ⅰ (carboxyl-terminal repeat domain kinase,CTDK-Ⅰ)由CTK1、CTK2和CTK3组成,作用于RNA聚合酶Ⅱ羧基端结构域,动态磷酸化CTD的七肽重复序列(YSPTSPS)来调控转录和翻译。酵母中的特异性蛋白CTK3与特殊的细胞周期蛋白CTK2结合形成异二聚体,再与CTDK-Ⅰ的催化亚基CTK1结合以调节其活性。CTK1作为细胞周期蛋白CDK (cyclin dependent kinase,CDK)的同源蛋白,其结构与功能的研究可拓展人们对CDK蛋白家族的认识;CTK2-CTK3复合物对CTK1调控机制的研究也可为细胞周期蛋白抑制剂的研发提供新的思路。本文简述了酵母CTDK-Ⅰ的功能特点及其亚基的结构与功能以及亚基间的相互作用,并展望了CTDK-Ⅰ复合物的研究前景。
Carboxyl-terminal repeat domain(CTD) of RNA polymerase Ⅱ largest subunit Rpb1 is essential for transcription regulation. Carboxyl-terminal repeat domain kinase(CTDK-Ⅰ) is composed of CTK1, CTK2 and CTK3, acting on RNA polymerase Ⅱ carboxyl-terminal repeat domain and phosphorylating CTD heptapeptide repeat(YSPTSPS) for regulating transcription and translation. The specific protein CTK3 binds to cyclin CTK2 to form a heterodimer, controlling CTK1 activity by binding to CTK1. Structural and functional study of CTK1, a homologous protein of cyclin dependent kinase(CDK), may provide a new idea for the research of CDK family, and analysis of the regulatory mechanism of activation of CTK1 by CTK2-CTK3 complex may offer an innovative method for developing cell cycle protein inhibitors. This article reviews the functional characteristics of CTDK-Ⅰ and the structures and interactions of its subunits and provides a useful guide for the studies of CTDK-Ⅰ complex in the future.
作者
朱文俊
毛雪玲
邱晓挺
Wenjun Zhu, Xueling Mao, Xiaoting Qiu(Key Laboratory of Applied Marine Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang Province, China)
出处
《微生物学报》
CAS
CSCD
北大核心
2018年第10期1701-1710,共10页
Acta Microbiologica Sinica
基金
国家自然科学基金(31400683)
宁波大学人才引进启动基金(013-E00843134702
013-E00843144702
013-421504460)
宁波大学王宽诚基金
宁波大学添路职业发展基金学生培育项目~~
关键词
细胞周期蛋白依赖性激酶
磷酸化
转录调控
亚基相互作用
earboxy-terminal repeat domain kinase
phosphorylation
transcription regulation
subunit interaction