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致仔猪腹泻沙门菌分离鉴定与耐药性分析

Isolation,identification and drug resistance analysis of Salmonella causing diarrhea in piglets
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摘要 为了鉴定致仔猪腹泻沙门菌并分析耐药性。从中牟地区规模化的养殖场采集患腹泻仔猪粪便、肠内容物等样品120份,通过分离鉴定的方法鉴定出57株沙门菌。人工感染小鼠致病试验结果表明,32株为致病性沙门菌。采用K-B药敏纸片法对32株致病性沙门菌进行药敏试验并分析耐药性,结果显示,32株致病性沙门菌对头孢曲松、头孢他啶、环丙沙星等4种药物耐药性在25. 0%~37. 5%之间;对氟苯尼考、阿米卡星、强力霉素等7种药物耐药性在40. 6%~78. 1%之间;对大观霉素、新霉素、阿莫西林等5种药物耐药性在87. 5%~100%之间。结果表明,从腹泻仔猪体内分离到32株致病性沙门菌对常用的抗生素具有很强的耐药性。 In order to identify Salmonella causing diarrhea in pigs and analyze drug resistance, 120 samples of feces and intestinal contents of diarrhoeal piglets were collected from large-scale farms in Zhongmu. 57 strains of Salmonella were identified by isolation and identification. The pathogenic test of artificially infected mice showed that 32 strains were pathogenic Salmonella. Drug suscepti- bility test and drug resistance analysis of 32 strains of Pathogenic Salmonella were carried out by K-B drug sensitive disk method, The results showed that 32 strains of Pathogenic Salmonella were resistant to 4 drugs such as ceftriaxone, ceftazidime, ciprofloxacin and drug resistance rate was between 25.0% - 37.5. They were resistance to 7 kinds of drug such as florfenicol, Amikacin, and doxy- cycline, and the drug resistance rate was 40. 6% - 78.1%. The resistance to 5 drugs such as dammycin, neomycin, amoxicillin was 87.5% - 100%. The results showed that 32 pathogenic Salmonella strains isolated from diarrhoea1 piglets were highly resistant to commonly used antibiotics.
作者 刘燕 LIU Yan(School of animal husbandry engineering,Henan Vocational College of Agriculture,Zhongmou 451450,China)
出处 《中国兽医杂志》 CAS 北大核心 2018年第6期96-98,共3页 Chinese Journal of Veterinary Medicine
基金 河南农业职业学院青年骨干教师培养计划项目(HNACKY-2018-01)
关键词 仔猪 腹泻 沙门菌 药敏试验 耐药性分析 piglets diarrhea Salmonella drug sensitivity test drug resistance analysis
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  • 1董世山,蔡辉益,刘作华,张世华,陈春林,刘国华,曹国文,刘宗慧.几种中药复方提取物对仔猪腹泻的防治作用及相关机理[J].中国农业大学学报,2005,10(3):60-64. 被引量:31
  • 2马孟根,王红宁,余勇,李成忠,张东,羊云飞,刘世贵.猪源致病性沙门氏菌耐药基因的分析[J].畜牧兽医学报,2006,37(1):65-70. 被引量:65
  • 3蒋培余,潘劲草.细菌遗传元件水平转移与抗生素抗性研究进展[J].微生物学通报,2006,33(4):167-171. 被引量:23
  • 4刘健华,陈杖榴.喹诺酮类抗菌药耐药新机制[J].中国兽医杂志,2007,43(1):54-57. 被引量:11
  • 5杨雪,郭定宗,祝海宝,吴庆儒,李文波,千国胜.某猪场断奶腹泻仔猪致病菌的调查[J].中国畜牧兽医,2007,34(8):114-116. 被引量:4
  • 6Ranh K,De Grandis S A,Clarke R C. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as specific method of detection of Salmonella[J].{H}MOLECULAR AND CELLULAR PROBES,1992,(4):271-279.
  • 7Li Yu,Fang Yan-Hong,Zhu Heng-Wen. The Analysis of ERIC-PCR Genomic Polymorphism of Salmonella Isolated Steains in Pig Careass[J].Journal of Animal and Veterinary Advances,2011,(13):1694-1698.
  • 8Edrington T S,Ross T T,Callaway T R. Investigation into the seasonal samonellosis in lactating dairy cattle[J].{H}Epidemiology and Infection,2008,(3):381-390.
  • 9Funk J A,Davies P R,Nichols M A. The effect of fecal sample weight on detection of Salmonella enterica in swine feces[J].{H}Journal of Veterinary Diagnostic Investigation,2000.412-418.
  • 10Vimont A J M,Kiermeier A,Padula D J. The adequacy of sample type/weight and incubation period on detection of Salmonella spp in slaughter cattle[J].{H}Journal of Veterinary Diagnostic Investigation,2005.430-435.

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