枯草芽孢杆菌对铅暴露鲫鱼的代谢及保护作用

Metabolism and Protection effects of Bacillus subtilis against Pb toxicity in Crucian carp

为研究枯草芽孢杆菌WB600对不同浓度铅暴露下鲫鱼各组织内铅代谢及躯干肾抗氧化酶活力的影响。将实验鲫鱼分为对照Ⅰ组;益生菌对照Ⅱ组;铅暴露组(Ⅳ、Ⅵ、Ⅷ组水体铅浓度分别为0. 05 mg/L Pb、0. 5 mg/L Pb、1 mg/L Pb);益生菌治疗组(Ⅲ、Ⅴ、Ⅶ组分别为0. 05 mg/L Pb+WB600、0. 5 mg/L Pb+WB600、1 mg/L Pb+WB600)。60天后,检测鲫鱼脑、鳃、肝胰脏、躯干肾、肠及肌肉中铅含量及躯干肾CAT、SOD、GSH、T-AOC抗氧化酶活力变化。结果显示,与对照组相比,各组织铅蓄积量随水体铅浓度升高而升高。在0. 05 mg/L Pb浓度下,相比于铅暴露组,添加WB600组肝胰脏、躯干肾、肠及肌肉中铅含量均显著降低(P <0. 05),脑中铅含量虽有降低,但无显著性差异(P> 0. 05);伴随着水体铅浓度的增加,除鳃和肌肉外,添加WB600组脑、肝胰脏、躯干肾、肠中铅蓄积量均显著低于铅暴露组。相比于铅暴露组,添加WB600组躯干肾CAT、SOD、GSH及TAOC水平均得到提高。表明在长期低浓度铅暴露下,饲喂WB600可降低鲫鱼脑、鳃、肝胰脏、躯干肾、肠及肌肉中的铅蓄积量,提高机体抗氧化能力。

In order to study the effects of Bacillus subtilise WB600 on lead metabolism and antioxidant activity of trunk kidney in various tissues of Carassius aurous exposed to different concentrations of lead, the experimental crucial carp was divided into control group I ;probiotics control group I1 ;Lead exposure group（for IV, VI, and VS group water lead concentrations were 0.05 mg · L-1 Pb,0.5 mg · L-1Pb, and 1 mg · L-l Pb） ; probiotics treatment groups （ for III, V, VII groups, the lead was O. 05 mg · L- l Pb ＋ WB600,0.5 mg·L-1 Pb ＋ WB600,and 1 mg ·L-1 Pb ＋ WB600）. After 60 days, the levels of lead in brain, Gill, hepatopancreas, trunk kidney, intestine and muscle of carp and the changes of CAT, SOD, GSH and T - AOC in trunk kidney were analyzed. The re sults showd that ： compared with the control group, the content of lead in crucial carp tissues increased with the increase in lead con- centration in water. At 0.05 mg ~ L-~Pb concentration,the content of lead in hepatopancreas, trunk kidney, intestine and muscle of WB600 group was decreased significantly when compared to lead exposure group （ P 〈 0.05 ）. Although the content of lead in the brain was decreased, there was no significant difference （P 〉 0.05） . With the increase of lead concentration in water, except gill and muscle, WB600 group had significantly lower levels of lead accumulation in brain, hepatopancreas, trunk kidney and intestine than those in lead exposure group. Compared with the lead exposure group, the levels of CAT, SOD, GSH and TAOC in the trunk kidney of the WB600 group was increased significantly. For the long-term low concentrations of lead exposure, feeding WB600 re- duced the carp brain, Gill, hepatopancreas, trunk kidney, intestine and muscle lead accumulation, and improved the body＇s antioxi- dant capacity.