四川兔源大肠埃希菌的耐药性及耐药基因检测

Antibiotic resistance and resistance genes detection of Escherichia coli in Sichuan rabbits

从四川地区规模化家兔养殖场分离鉴定出97株大肠埃希菌(Escherichiacoli),采用Kirby–Bauer纸片法进行耐药性测定,同时采用PCR方法对相关耐药基因进行检测。结果显示:分离菌株对四环素类药物表现高度耐药,耐药率高达90.72%以上,对磺胺类药物的耐药率达72.16%,对氨基糖苷类药物的耐药率31.96%~58.76%,对多肽类的耐药率较低,为14.43%;耐药基因tet A、tet B、tet C、arm A、rmt B和flo R的检出率分别为61.86%、84.54%、16.49%、4.12%、3.09%和8.25%,未检出tet D、tet E、tet M、tet O、tet L、tet K、rmt A、rmt C、rmt D、rmt E、npm A和cfr基因。

97 Escherichia coli were isolated from rabbits farms in Sichuan and subjected to detections of antibiotics resistance and resistance genes by K–B disk diffusion and PCR. The results revealed that the isolated strains had highest resistance to tetracyclines with the rate over 90.72%, the resistance sulfonamides with the rate of 72.16%, the resistance to aminoglycoside with the rate between 31.96%–58.76%, the resistance polymyxin B with lowest rate of 14.43%. The detected genes and their rates were tetA of 61.86%, tetB of 84.54%, tetC of 16.49%, armA of 4.12%, rmtB of 3.09% floR of 8.25%. And, no tetD, tetE, tetM, tetO, tetL, tetK, rmtA, rmtC, rmtD, rmtE, npmA, cfr was detected.