刺玫果总皂苷的纯化工艺及体外活性研究

Study on the Purification Method of Total Saponins from Fruit of Rosa Davuvrica Pall and its Purification Method

研究不同型号树脂对刺玫果总皂苷的纯化效果及刺玫果总皂苷体外活性.分别采用LSA-21、D-101和AB-8等8种型号的大孔树脂对刺玫果总皂苷进行纯化,通过紫外-可见分光光度法测定吸光度,从而计算吸附率和解吸率吸附率和解吸率.结果表明,LSA-21型大孔树脂对对刺玫果总皂苷的纯化效果最好,其最佳工艺条件为:吸附液浓度为0.192 7 mg/mL,吸附液体积为25 mL,吸附液pH为6.5,乙醇体积分数为90%,解吸液体积为40 mL,解吸液pH为8.5.在上述纯化条件下,刺玫果总皂苷的吸附率、解吸率分别可以达到77.51%和65.28%,干浸膏中总皂苷的含量由9.49%提高到33.65%.试验结果表明,刺玫果总皂苷对DPPH·、·OH、超氧阴离子自由基、ABTS自由基均具有清除能力,同时具有一定的体外抗氧化活性,并能够抑制脂质和α-葡萄糖苷酶的活性,试验结果为刺玫果总皂苷的进一步研究提供了参考.

The purification effect of different models resin and their activity in vitro of the total saponins in fruit of Rosa davuvrica Pall.were studied.Eight types of macroporous resins,such as LSA-21,D-101 and AB-8,were used to purify the total saponins from Fruit of Rosa davuvrica Pall.Adsorption rate and desorption rate were determinated by ultraviolet-visible spectrophotometry method.The results showed that the LSA-21 type of macroporous resin had the best purification effect on the total saponins in fruit of Rosa davuvrica Pall.The best conditions were as follows：The concentration of adsorption liquid was 0.192 7 g/mL.The volume of adsorption liquid was 25 mL.The pH of adsorption liquid was 6.5.The volume fraction of ethanol was 90%.The volume of desorption liquid was 40 mL and the desorption solution pH was 8.5.With the above purification conditions,the calculated adsorption and desorption rate of the total saponins in fruit of Rosa davuvrica Pall.could reach 77.51 % and 65.28%,respectively.The content of the total saponins in dry extract was increased from 9.49% to 33.65%.The test results showed that scavenging ability to DPPH·,·OH,superoxide anion and ABTS free radicals,vitro antioxidant activity,and the inhibition of the activity of alpha glucosidase can be benefited from the total saponins in fruit of Rosa davuvrica Pall..The results provide a reference to further study on the total saponins in fruit of Rosa davuvrica Pall.