HPLC法同时测定五味藿香片中6种成分

Simultaneous determination of six constituents in Wuwei Huoxiang Pills by HPLC

目的建立HPLC法同时测定五味藿香片(广藿香、陈皮、草豆蔻等)中芹菜素-7-O-β-D-吡喃葡萄糖苷、橘皮素、橙皮苷、川陈皮素、香草酸、原儿茶酸的含有量。方法该药物甲醇提取液的分析采用Agilent C_(18)色谱柱(4. 6 mm×250 mm,5μm);流动相乙腈-0. 1%磷酸,梯度洗脱;体积流量1. 0 mL/min;检测波长270 nm;柱温30℃。结果 6种成分在各自范围内线性关系良好(r> 0. 999 0),平均加样回收率96. 6%~98. 1%,RSD 0. 47%~2. 73%。结论该方法准确可靠,重复性好,可用于五味藿香片的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of apigenin-7-O-β- D-glucopyranoside, tangeretin, hesperidin, nobiletin, vanillic acid and protocatechuic acid in Wuwei Huoxiang Pills （Pogostemonis Herba, Citri reticulatae Pericarpium, Alpiniae katsumadai Semen, etc. ）. METHODS The analysis of methanol extract of this drug was perforated on a 30℃ thermostatic Agilent C18 scolunm （4.6 mm×250 ram, 5 μm） , with the mobile phase comprising of acetonitrile-0. 1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 270 nm. RESULTS Six constituents showed good linear relationships within their own ranges （r 〉 0. 999 0） , whose average recoveries were 96.6% - 98.1% with the RSDs of 0.47% - 2.73% . CONCLUSION This accurate, reliable and reproducible method can be used for quality control of Wuwei Huoxiang Pills.