白术总黄酮提取工艺优化及其抗氧化活性的研究

Optimization of extraction technology of total flavonoids and its anti-oxidation from Atractylodes macrocephala

以白术为实验对象,采用响应面法对白术总黄酮的提取工艺进行优化,并探究了白术总黄酮的抗氧化活性。通过Design-Expert响应面软件确定了最佳提取工艺参数:提取温度79. 1℃,液料比31. 5∶1,时间1. 91 h。在此条件下,响应面软件的预测吸光度值为0. 239 4,五组验证实验的平均吸光度为0. 242 2,相对误差仅为2. 01%,R2=0. 996 5显示该回归模型可准确地预测贝母中皂苷的提取量。白术黄酮具有较好的抗氧化活性,清除效果依次为ABTS自由基>羟基自由基>超氧根离子自由基> DPPH自由基>金属离子自由基。

The extraction technology of total flavonoids from Atractylodes macrocephala was optimized by response surface methodology, and the antioxidant activity of total flavonoids from Atractylodes macrocephala was studied. The optimum extraction parameters were determined by Design-Expert response surface software： the extraction temperature was 79.1 ℃, the liquid to material ratio was 31.5∶1, and the time was 1.91 h. Under this condition, the predicted absorbance value of the response surface software is 0.239 4, the average absorbency of the five groups is 0.242 2, and the relative error is only 2.01%. The R 2 =0.996 5 model shows that the regression model can accurately predict the extraction of saponins in Fritillaria. The flavonoids of Atractylodes macrocephala have good antioxidant activity, and the effect of scavenging is ABTS radical 〉 hydroxyl radical 〉 superoxide radical ion radical 〉DPPH radical 〉 metal ion radical.