子宫颈鳞状细胞癌组织中泛素特异性蛋白酶4和基质金属蛋白酶2的表达及意义

Expression and clinical significance of USP4 and MMP2 in cervical squamous cell carcinoma tissues

目的观察宫颈鳞状细胞癌及癌旁正常宫颈组织中泛素特异性蛋白酶4(USP4)和基质金属蛋白酶2(MMP2)的表达,探讨两者在宫颈鳞状细胞癌发生、发展中的作用及相关性,并分析两者与各临床病理因素的关系。方法收集常州市金坛区人民医院病理科2010年1月至2013年12月70例宫颈鳞状细胞癌组织及癌旁组织,采用免疫组化检测USP4和MMP2的表达。结果 USP4和MMP2在宫颈鳞状细胞癌中的表达率分别为61.4%,44.3%,而在癌旁组织中的表达分别为8.0%,4.3%。它们在有淋巴结转移的宫颈癌组织中表达率分别为80.0%,90.7%,在无淋巴结转移的宫颈癌组织的表达分别为47.5%,56.1%。在浸润深度≥1/2间质的宫颈癌组织中的表达分别为62.2%,83.8%,在<1/2间质的宫颈癌组织中的表达为29.2%,37.5%,与淋巴结转移及浸润深度呈正相关(P<0.05),但与患者年龄、肿瘤大小、组织学分级无关(P>0.05)。且USP4和MMP2在宫颈鳞癌组织中的表达呈正相关(P<0.05)。结论 USP4和MMP2表达可能与宫颈鳞状细胞癌的发生发展有关,可作为预后判断和治疗指导的参考指标之一。

Objective To evaluate the expressions of ubiquitin-specific protease 4（ USP4） and matrix metalloproteinases 2（ MMP2）in cervical squamous cell carcinoma tissues and normal cervical tissues,to discuss their relevance and roles in carcinogenesis and development of cervical squamous cell carcinoma,and to investigate their correlation with the clinicopathological features. Methods Immunohistochemical technique was used to detect the expression of USP4 and MMP2 in 70 cases of cervical squamous cell carcinoma tissues and normal cervical tissues collected from Jintan People＇s Hospital between January 2010 and December 2013. Results The expression rates of USP4 and MMP2 in the cervical squamous cell carcinoma tissues were 61. 4%,44. 3%,and the expression rates were 8. 0%,4. 3% in the normal cervical tissues. The expression rates of USP4 and MMP2 in the cervical squamous cell carcinoma tissues with lymph node metastasis were 80. 0%,90. 7%,while the expression rates were 47. 5%,56. 1% in those without lymph node metastasis.The expression rates were 62. 2%,83. 8% in those cervical carcinoma tissues deeper than or equal to 1/2 interstitium,and the expression rates were 29. 2%,37. 5% in those less than 1/2 interstitium. They were related to lymph node metastasis and the depth of invasion（ P〈0. 05）,but not related to age,tumor size,and differentiation（ P〈0. 05）. There was a positive correlation between USP4 and MMP2 in the cervical squamous cell carcinoma（ P〈0. 05）. Conclusion The expressions of USP4 and MMP2 may contribute to the carcinogenesis and development of cervical squamous cell carcinoma,which could be a marker to evaluate prognosis and guide treatment.