HLA-G在乳腺癌中的表达及意义

The expression of HLA-G in breast cancer and its significance

目的探讨乳腺癌患者组织中人类白细胞抗原G(Human leukocyte antigen,HLA-G)的表达及其在乳腺癌诊断和预后中的作用。方法分离纯化HLA-G蛋白,筛选出分泌抗HLA-G单克隆抗体的杂交瘤细胞株,得到抗HLA-G单克隆抗体。用免疫组织化学方法检测235例乳腺癌患者组织中HLA-G的表达,分析HLA-G的表达与各项临床病理指标、宿主免疫反应及生存率之间的关系。结果 HLA-G在乳腺癌患者的表达率为66%(155/235),HLA-G表达与肿瘤大小、临床TNM分期以及淋巴结转移有关。HLA-G阴性病人的生存率明显高于HLA-G阳性病人。结论乳腺癌组织中HLA-G有较高表达,HLA-G的表达与宿主免疫反应及乳腺癌的预后有关,HLA-G的表达可能是影响乳腺癌病人预后的独立因素。

Objective The Objective of this study was to investigate the expression of human leukocye antigen-G（HLA-G）in breast cancer patients and its role in the diagnosis and prognosis of breast cancer.Methods HLA-G protein was isolated and purified,and hybridoma cell lines secreting anti-HLA-G monoclonal antibody were screened to obtain anti-HLA-G monoclonal antibody.The expression of HLA-G in 235 primary breast cancer tissues was detected by immunohistochemistry.The relationship amongst HLA-G expression with various clinicopathological parameters,host immune response ＆ survival rate was analyzed.Results The expression rate of HLA-G in breast cancer patients was 66%（155/235）.The expression of HLA-G was related to tumor size,clinical TNM stage and lymph node metastasis.The survival rate of HLA-G negative patients was significantly higher than that of HLA-G positive patients.Conclusion HLA-G is highly expressed in breast cancer tissues.The expression of HLA-G is related to host immune response and prognosis of breast cancer.HLA-G is an independent factor affecting the prognosis of breast cancer patients.