超高效液相色谱-串联质谱法测定禽蛋氟喹诺酮类药物残留的不确定度分析

Uncertainty Evaluation of Determination of Fluoroquinolones Residues in Poultry Eggs by UPLC-MS/MS

为了研究超高效液相色谱-串联质谱法(LC-MS/MS)测定禽蛋氟喹诺酮类药物的不确定度,对影响超高效液相色谱-串联质谱法测定禽蛋氟喹诺酮类药物的不确定度因素进行了分析与评定,依次计算影响测定结果的各变量的不确定度、合成不确定度和扩展不确定度。结果表明:当达氟沙星含量为4.334μg/kg时,其扩展不确定度为0.871 4 (k=2);当恩诺沙星含量为4.458μg/kg时,其扩展不确定度为0.456 9 (k=2)。LC-MS/MS法测定禽蛋氟喹诺酮类药物含量的不确定度主要是标准曲线拟合引入和添加回收引入。

The uncertainty of determination of fluoroquinolones residues in poultry eggs by UPLC-MS/MS was analyzed and evaluated. The uncertainty,synthetic uncertainty and expanded uncertainty of each variable were calculated. The results showed that when the content of danofloxacin was 4.334 μg/kg,the expanded uncer-tainty was 0.871 4 （k=2）,and when the content of enrofloxacin was 4.458 μg/kg,the expanded uncertainty was 0.456 9 （k =2） . The preparation of standard solution,curve fitting and measurement repeatability were the main sources of uncertainty.