原花青素对糖尿病大鼠肾的保护作用

Protection of Proantho cyanidins on Kidney in Diabetic Rats

本文主要研究了原花青素(PC)对糖尿病大鼠肾脏的保护作用,并初步探讨其作用机制。实验中采用高脂高糖饲料喂养联合链脲佐菌素(STZ)损伤建立糖尿病大鼠模型。将造模大鼠按照体重随机分为模型组和实验组,同时设立正常组。实验组,每日腹腔注射原花青素150 mg/kg,其余2组腹腔注射等体积的生理盐水,连续给药4周后,检测血糖(BG)、总胆固醇(TC)、三酰甘油(TG)及肾脏组织中丙二醛(MDA)、蛋白羰基化(PCO)、超氧化物歧化酶(SOD)及过氧化氢酶(CAT)的活性,光镜观察肾组织结构。结果表明,与正常组相比,模型组TC、TG显著升高了4.02倍和6.88倍(p<0.01),肾脏组织中的SOD活性降低27.88%,CAT活性降低了17.61%(p<0.01),肾脏组织MDA、PCO含量显著升高了51.10%和81.36%(p<0.01);与模型组相比,实验组大鼠血糖、TC和TG水平明显降低了50.84%、55.70%和70.52%(p<0.01),肾脏组织中的SOD、CAT活性明显升高了26.05%和12.97%(p<0.01),肾脏组织MDA、PCO含量显著降低到21.54%和11.86%(p<0.01)。病理切片显示,PC保护可减轻STZ引起的肾小球萎缩等病理损伤。由结果可知,原花青素对糖尿病大鼠肾具有一定的保护作用,其机制可能与其抗氧化作用有关。

This study aims to explore the protective effect of Proantho cyanidins（PC） on diabetic rats kidney and potential mechanism. In this work, the diabetic rats model was established by feeding with a high fat and sugar diet in combination with a streptozotocin treatment. Animals were randomly divided into three groups： model group, test group（PC, 150 mg/kg） and normal group. After 4 weeks of treatment, blood glucose, total cholesterol（TC）, triglyceride（TG） and kidney malondialdehyde（MDA）, protein carbonyl（PCO）, superoxide dismutase activities（SOD） and catalase（CAT） activities were determined. Results showed that as compared with the normal group, TC and TG in the model group were significantly increased by 4.02-fold and 6.88-fold（P〈0.01）, while the SOD and CAT activities in the kidney tissue were significantly（P〈0.01） decreased by 27.88% and 17.61%, respectively. The levels of MDA and PCO increased significantly by 51.10% and 81.36%（P〈0.01）. As compared with the model group, the blood glucose, TC and TG levels in the experimental group were significantly reduced by 50.84%, 55.70% and 70.52%, respectively（P〈0.01）. The activities of SOD and CAT in kidney tissues were significantly increased by 26.05% and 12.97%（P〈0.01）, and the contents of MDA and PCO in kidney tissues were significantly decreased to 21.54% and 11.86% l, respectively（P〈0.01）. The pathologic slice revealed that PC pretreatment could reduce STZ-induced pathological damage such as glomerular atrophy. These results demonstrated that PC may exhibit a protection on diabetic rats kidney, and the underlying mechanism might be related to its anti-oxidative properties and free radical scavenging abilities.