重庆地区尖锐湿疣患者HPV检测及基因分型分析

Detection and Genotyping of Human Papillomavirus in Patients with Condyloma Acuminatum in Chongqing

目的探讨重庆地区人类乳头瘤病毒(HPV)感染的分子流行病学特点,从而为本地区尖锐湿疣(CA)患者的监测及临床治疗、预防提供一定的临床依据。方法采用基因芯片法对重庆地区401例CA患者的皮损组织进行HPV检测及分型。结果 401例患者中384例检测出HPV阳性,共发现23种HPV亚型,其中HPV6型感染占46. 88%(180/384),HPV11感染占40. 89%(157/384)。其中单一感染226例,占比58. 85%(226/384)。158例存在2~6种亚型多重感染,多重感染率为41. 15%(158/384),其中三重感染占9. 38%(36/384),以HPV6+11+43感染多见;四重感染者共有16例,占4. 17%(16/384);五、六重感染者全为男性,占1. 56%(6/384)。多重感染中以高低危型HPV混合感染为主。结论重庆地区尖锐湿疣以低危型HPV6、11感染为主,高危型HPV感染发生率高,并多以高低危混合感染形式存在。

Objective To explore the molecular and epidemiological characteristics of human papillomavirus（HPV） infection in patients with Condyloma Acuminatum（CA） in Chongqing. Methods HPV genotypes were detected with gene chip methods in patients with CA. Results It showed that 384 out of 401 samples were HPV positive.23 subtypes were found,and they were mainly HPV6 type（46. 88%,180/384） and HPV 11 type（40. 89%,157/384）. The single type of HPV was 226 cases with the rate of 58. 85%（226/384）. A total 158 cases with multiple infections and the rate was 41. 15%. Trebling infection accounted for 9. 38%,mainly with HPV6 ＋ 11 ＋ 43 infection. Fourfold infection was 16 cases（4. 17%）. All five or six fold infections were male,accounted for 1. 56%. The mixed infection was the main type of multiple infections. Conclusion The infection of HPV in patients with CA in Chongqing is mainly low risk type of HPV（6,11）,and the infection with high risk type is oftenfound in the mixed infection with multiple HPV types.