斜叶黄檀精油功能活性

Functional Activity of Esential Oil from Dalbergia pinnata( Lour.) Prain.

本文主要测定斜叶黄檀精油中的挥发性成分、多酚和黄酮含量,采用体外抗氧化评价斜叶黄檀精油的清除DPPH和ABTS自由基能力、总还原力和总抗氧化能力,以金黄色葡萄球菌(G+)、化脓性链球菌(G+)、大肠杆菌(G-)、铜绿假单胞菌(G-)、鼠伤寒沙门氏菌(G-)以及白色念珠菌(真菌)为待测菌,评价精油的抑菌效果,通过对酪氨酸酶的抑制活性评价精油抑制黑色素的形成能力。结果表明斜叶黄檀精油在浓度为0.18 mg/m L时,对DPPH和ABTS自由基的清除能力均达到70%左右,在浓度分别为0.18 mg/m L和1 mg/m L时,总还原力和总抗氧化能力的吸光度值均达到0.4以上。对金黄色葡萄球菌、化脓性链球菌以及白色念珠菌的抑菌圈直径分别为:12.83、11.50和10.25 mm,呈现中敏程度的抑制性,但对三种革兰氏阴性菌无明显的抑制效果。精油对酪氨酸酶的抑制活性要强于阳性对照熊果苷,在浓度为0.1 mg/m L抑制率将近90%。本文得出斜叶黄檀精油有较好的抗氧化活性、抑制革兰氏阳性菌和真菌以及抑制酪氨酸酶活性,比较完善的介绍了斜叶黄檀精油的功能活性。

In this paper,the main volatile components,the content of polyphenols and flavonoids in the essential oil of Dalbergia pinnata（ Lour.） prain. was determined,and the antioxidant,antibacterial and inhibit tyrosinase activities of essential oils were studied.The antioxidant activities of esential oil from Dalbergia pinnata（ Lour.） Prain was evaluated by the free radical scavenging of DPPH and ABTS,total reducing power and total antioxidant capacity. Staphyloccocus aureus Rosenbach ATCC25923,Streptococcus pyogenes ATCC 19615,Escherichia coli CMCC（ B） 44103,Pseudomonas aeruginosa ATCC 27853,Salmonella Typhimurium ATCC 14028,Candida albicans ATCC 10231 were used as tested bacteria to evaluate the antibacterial effect of essential oil.The inhibitory effect of essential oil on the formation of melanin was evaluated by the inhibits of tyrosinase activity.The results showed that the DPPH and ABTS free radical scavenging capacity reached about 70% at the concentration of 0.18 mg/mL,at the concentrations of 0.18 mg/mL and 1 mg/mL,the absorbance values of total reducing power and total antioxidant capacity reached 0.4 or more. The inhibition zone diameters of Staphyloccocus aureus Rosenbach,Streptococcus pyogenes and Candida albicans were： 12.83,11.50,and 10.25 mm,respectively,exhibiting moderate-inhibitory degrees,but there was no obvious inhibitory effect on the three Gram-negative bacteria. Even the inhibitory activity against tyrosinase was stronger than that of the positive control arbutin,and the inhibition rate was nearly 90% at a concentration of 0.1 mg/mL.This article concluded that Dalbergia pinnata（ Lour.） prain. essential oil have good antioxidant activity,inhibits Gram-positive bacteria and fungi,and inhibit tyrosinase activity,described the complete functional activities of essential oil from Dalbergiapinnata（ Lour.） Prain.