多孔钛片表面ZnO薄膜的表征及其对腐败希瓦氏菌生物被膜的抑制性能

The Characterization of Zinc Oxide Films on the Surfaces of Porous Titanium Substrates and Their Antibiofilm Properties on Shewanella putrefaciens

为研究ZnO薄膜微观形貌对其抑制腐败希瓦氏菌生物被膜性能的影响,采用水热合成法制备了ZnO薄膜,并对其进行X射线衍射(XRD)、扫描电子显微镜(SEM)和水接触角表征分析。结果表明,Zn O为六方纤锌矿晶体结构,水热反应时间影响ZnO薄膜的微观形貌。延长水热反应时间,薄膜表面ZnO均匀堆积,其附着量不断增加。多孔钛片呈疏水性,水热合成ZnO薄膜呈亲水性。ZnO薄膜表面的生物被膜附着量随菌体培养时间延长而增加。0~2 h,Zn O薄膜表面生物被膜粘附率和被膜菌总数上升很快,菌体在生物被膜内迅速繁殖; 2~12 h,生物被膜粘附率和被膜菌总数持续增长,生物被膜进入生长期; 12~36 h,被膜粘附率及被膜菌菌落总数变化较小,生物被膜进入成熟期; 36 h后,生物被膜粘附率和被膜菌总数下降,生物被膜进入衰退期。由于ZnO具有杀菌性能,Zn O薄膜对生物被膜的抑制性能增强,且制备ZnO薄膜的水热反应时间越长,样品对生物被膜的抑制性能越优。ZnO杀菌性能对材料表面抗生物被膜粘附性能的贡献远优于材料表面的疏水性能。

To study the influence of the morphologies of ZnO films on its antibiofilm properties for Shewanella putrefaciens,Zn O films were prepared by hydrothermal synthesis method,which were characterized by X-ray diffraction（ XRD）,scanning electron microscope（ SEM） and water contact angle. The results showed that Zn O was hexagonal wurtzite structure,and the microstructures of ZnO films were affected by hydrothermal time.Extension of hydrothermal time,the Zn O particles were evenly stacked on the surface,and adhesion amount were increased continuously. The porous titanium film was hydrophobic,however the ZnO films exhibited hydrophilic. The attached biofilm on the ZnO films surface was increased with the incubation time.Between 0 - 2 h,the biofilm adhesion rate and total number of bacteria were increased rapidly,and bacteria were rapidly proliferated in the biofilm.Between 2-12 h,the biofilm adhesion rate and total number of bacteria were increased continuously,and the biofilm entered the growth phase.Between 12-36 h,the biofilm adhesion rate and total number of bacteria were held steady,and the biofilm entered mature stage.After 36 h,the biofilm adhesion rate and total number of bacteria were declined,and the biofilm entered recession period. The antibiofilm property of the ZnO film was improved due to its antibacterialproperty,and the longer the hydrothermal reaction time,the better the antibiofilm property was. The contribution of the bactericidal effect of ZnO films against the biofilm adhesion performance was far better than its hydrophobic properties.