甘肃传统浆水发酵过程中产双戊烯乳酸菌的分离筛选

Isolation and Identification of Producing-dipentene Lactic Acid Bacteria from the Traditionally Fermented Jiangshui in Gansu

目的:从传统发酵浆水中分离筛选产双戊烯乳酸菌株,以期为传统浆水工艺现代化改造过程增香发酵剂的开发提供菌株来源。方法:采用经典的平板分离筛选方法初筛菌株,结合发酵性能测定复筛。结果:从甘肃传统发酵浆水中初筛得到5株产双戊烯乳酸菌,通过测定其发酵产双戊烯性能,从中复筛得到1株产双戊烯乳酸菌菌株R-32,该菌在马铃薯番茄液体培养基中产双戊烯含量可达到0.171μg/m L;通过观察其形态、测定生理生化指标及进行产乳酸的定性试验,鉴定该菌株R-32为肠膜明串珠菌属(Leuconostoc mesenteroides)。结论:本实验获得了一株可用于传统浆水发酵的产香乳酸菌株。

Objective： Isolate and identify producing-dipentene lactic acid bacteria from the traditionally fermented Jiangshui in order to provide strain source for the aroma-enhancing starter development of Jiangshui industry production.Methods：Preliminarily isolate lactic acid bacteria strains by classical plate separation method and screen highly producing-dipentene lactic acid bacteria with fermentation performance measurement.Results： The results showed that five strains of producing-dipentene lactic acid bacteria were obtained by preliminarily isolation from the traditionally fermented Jiangshui in Gansu. By measuring the fermentation performance of theirs production of dipentene,a strain lactic acid bacteria,named R-32,was obtained,which produced dipentene up to 0.171 μg/m L in fermentation liquid medium（ potato and tomato etc.）. It was identified as Leuconostoc mesenteroides by observing its morphology,evaluating physiological and biochemical characteristics including testing lactic acid production.Conclusion： In this research,a strain of aroma-producing lactic acid bacteria was obtained,which could be used in traditional Jiangshui fermentation.