不同种质地黄块根菊花心与非菊花心部位有效成分特征分析

Feature Analysis of Active Principle Between Radial Striations( Xylem) and Un-radial Striations( Phloem and Periderm) in Tuberous Root of Different Germplasms Rehmanniae Radix

目的：比较沁怀,85-5,北京1号,QH-1,1706,白选6种地黄生育期内菊花心与非菊花心中梓醇,毛蕊花糖苷,地黄苷A,D,益母草苷,多糖等指标性成分的含量,分析不同地黄种质资源菊花心的成分差异及质量特征,为地黄的质量评价及道地性揭示提供科学依据。方法：梓醇、毛蕊花糖苷含量测定参考2015年版《中国药典》;地黄苷A,D及益母草苷含量测定采用HPLC法,Dikma Diamonsil C_（18）色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-水（4∶96）,流速1 mL·min^（-1）,检测波长205 nm,柱温30℃,进样量20μL;多糖含量测定采用苯酚-硫酸法。结果：不同种质地黄块根各指标性成分在菊花心与非菊花心部位富集量差异很大。85-5与1706地黄菊花心部位中梓醇含量远高于非菊花心部位,而北京1号与QH-1地黄则是非菊花心部位明显高于菊花心部位;85-5,1706及QH-1地黄中毛蕊花糖苷在菊花心部位中的含量明显高于非菊花心部位,其他3个种质分布相对均匀;沁怀和北京1号地黄块根非菊花心部位中地黄苷A的含量明显高于菊花心部位;85-5,北京1号,1706地黄块根非菊花心部位中地黄苷D的含量均明显高于菊花心中的含量,其他种质差异不大;85-5,白选和1706地黄菊花心与非菊花心部位益母草苷含量差异不大,而北京1号,沁怀,QH-1地黄则为菊花心部位远高于非菊花心部位,差异达2~4倍;6种地黄块根非菊花心部位中的多糖含量明显高于菊花心部位。结论：种质在一定程度上决定了地黄块根次生代谢产物在地黄菊花心与非菊花心部位的积累与分布。

Objective： To Compare the content of catalpol,acteoside,rehmaionoside A,rehmaionoside D,leonuride and polysaccharide between radial striations and non-radial striations of Rehmannia glutinosa during growth with six germplasms（ Qinhuai,85-5,Beijing^（-1）,QH-1,1706,Baixuan）,and analyze the component differences and quality characteristics of radial striations in different germplasm resources of R. glutinosa,in order to provides a reference for quality evaluation and geoherbalism of R. glutinosa. Method： The method to determine the contents of catalpol and acteoside consulted Chinese Pharmacopoeia 2015 edition. The contents of rehmaionoside A,rehmaionoside D and leonuride were determined by HPLC. Chromatographic column was Dikma Diamonsil C_（18）（ 4. 6 mm × 250 mm,5 μm）,with mobile phase of acetonitrile-water（ 4 ∶ 96）; flow rate was 1. 0 mL·min^（-1）;detection wavelength was 205 nm; column temperature was 30 ℃; sample size was 20 μL. The content of polysaccharide was determined by phenol sulfate method. Result： There were significant differences in accumulation amount between radial striations and non-radial striations,particularly in tuberous root with different germplasms R. glutinosa. The content of catalpol in radial striations was far higher than that in non-radial striations of 85-5 and 1706,whereas that in non-radial striations was far higher than that in radial striations of Beijing^（-1） and QH-1. The content of acteoside in radial striations was far higher than that in un-radial striations of 85-5,1706 and QH-1,but with a relatively uniform distribution of the other three germplasms. The content of rehmaionoside A in non-radial striations was higher than that in radial striations evidently of Qinhuai and Beijing^（-1）. The content of rehmaionoside D in non-radial striations was higher than that in radial striations,but it was substantial consistent with the other three germplasms. The content of leonuride was substantial consistent with radial striations and nonradial striations of 85-5, Baixuan,1706; but the content in radial striations was far higher than non-radial striations of Qinhuai,Beijing^（-1）,QH-1,with up to 2-4 times of differences. The content of polysaccharide in nonradial striations was higher than that in radial striations of all six germplasms. Conclusion： The accumulation and distribute of secondary metabolite between radial striations and non-radial striations in the tuberous root of R. glutinosa were decided by germplasms resource to a certain degree.