吉氏巴贝斯虫人工感染对犬免疫功能的影响

Effect of artificial infection with Babesia gibsoni on canine immune function

通过静脉接种试验犬建立人工感染吉氏巴贝斯虫试验模型,利用MTT法检测外周血淋巴细胞（PMBC）的增殖能力,利用流式细胞术分析外周血中CD3~＋、CD4~＋、CD8~＋T淋巴细胞亚群的分布,利用Real-time PCR检测外周血中IL-6、IL-17、TNF-α、IFN-γmRNA的转录水平。结果显示,与对照组相比,感染吉氏巴贝斯虫后犬PMBC的增殖能力显著下降（P〈0.05）,外周血中CD3~＋、CD4~＋T淋巴细胞占比降低显著（P〈0.05）,CD8~＋T淋巴细胞占比下降不显著（P〉0.05）。与对照组犬相比,IL-6、TNF-α、IFN-γmRNA转录水平显著下调（P〈0.05）。结果表明,吉氏巴贝斯虫感染对犬免疫机能具有明显的抑制作用。

The experimental model of Babesia gibsoni artificial infection in dogs was established by intravenous inoculation.The proliferation of peripheral blood lymphocytes（PMBC） was detected by MTT assay.The distribution of CD3~＋,CD4~＋,CD8~＋T lymphocyte subsets in peripheral blood was analyzed by flow cytometry.And the transcription level of IL-6,IL-17,TNF-α and IFN-γ in peripheral blood of dogs was detected by real-time PCR.The results showed that the in vitro proliferation of PMBC of the dogs was significantly decreased compared with the control group（P〈0.05）,the proportion of CD3~＋ and CD4~＋T lymphocytes in the peripheral blood was significantly decreased（P〈0.05）,and the decrease of CD8~＋cells was not significantly（P〉0.05）.Compared with control dogs,the transcription levels of IL-6,TNF-α,and IFN-γ m RNA were down-regulated（P〈0.05）.The results show that B.gibsoni infection has a significant inhibitory effect on canine immune function.