延龄草总皂苷对宫颈癌细胞Hela裸鼠移植瘤的增殖抑制作用

Growth inhibition of Trillium saponins on xenografts of cervical cancer Hela cells in nude mice

目的观察延龄草总皂苷对宫颈癌细胞Hela裸鼠皮下移植瘤的影响及相关机制。方法培养宫颈癌细胞Hela,接种至BALB/c裸鼠背部皮下。接种后第5日通过腹腔注射的方式给予裸鼠延龄草总皂苷治疗,每隔3 d测量移植瘤的体积以及裸鼠的体重;实验结束时,采用TUNEL试剂盒检测移植瘤细胞的凋亡情况;Western blot法检测凋亡相关蛋白活化型caspase 3,8,9表达的变化。结果延龄草总皂苷可有效地抑制裸鼠移植瘤的增殖,实验结束时,肿瘤的体积分别为:模型组(1233.67±155.32)mm^3,延龄草总皂苷5 mg·kg^(-1)组(570.18±56.26)mm^3,延龄草总皂苷10 mg·kg^(-1)组(254.33±52.36)mm^3。延龄草总皂苷能诱导移植瘤细胞的凋亡,可浓度依赖性地促进细胞活化型caspase 3,9的表达。结论延龄草总皂苷可有效地抑制Hela裸鼠移植瘤的增殖,其可能机制是延龄草总皂苷通过caspase依赖的方式诱导Hela细胞发生凋亡。

Objective To detrmine the effect of Trillium saponin on the growth of xenografts of Hela cell in nude mice and the possible mechanism. Methods Hela cells were cultured and inoculated subcutaneously to the back of BALB/c nude mice. Five days later, the nude mice were intraperitoneally injected Trillium saponins. Tumor volume and body weight were measured every 3 days to the end of the experiment. TUNEL assay was used to detect the apoptosis of tumor cells; Western blot was employed to detect the expression of apoptosis related protein caspase 3, 8, 9. Results Trillium saponin inhibit the growth of xenografts of Hela cells significantly. At the end of the experiment, the tumor volume as follows： the model group （1233.67 ± 155.32） mm3, the Trillium saponin （5 mg·kg-1） （570.18±56.26） mm3, and the Trillium saponin （10 mg·kg^-1） group （254.33±52.36） mm3. Trillium saponins induced the apoptosis of tumor cells and promoted the expression of activated caspase 3, 9 in a dose-dependent manner. Conclusion Trillium saponin may suppress the growth of xenografts of Hela cell, at least in part, by inducing the apoptosis of tumor cells in a caspase-dependent way.