丹参酮ⅡA对缺血性心力衰竭大鼠心肌纤维化的影响

Tanshinone ⅡA Inhibits Myocardial Fibrosis in Rats with Ischemic Heart Failure

目的研究丹参酮ⅡA通过抑制Galectin-3表达对缺血性心力衰竭大鼠心肌纤维化的影响。方法 36只Wistar大鼠随机均分为3组:假手术组(Sham),缺血性心力衰竭组(IHF),丹参酮ⅡA组(TSA),建立缺血性心力衰竭模型,其中丹参酮ⅡA组于结扎冠脉前1周每天给予TSA预处理,剂量为40mg/kg,通过心脏彩超观察手术后4周各组心功能情况,通过Masson染色观察各组大鼠心肌纤维化程度,蛋白免疫印迹法测定Galectin-3蛋白表达情况。结果 TSA组与IHF组相比,心功能参数均出现显著改善(P均<0. 01); Masson染色示,TSA组明显降低了缺血性心力衰竭所造成的大片胶原纤维增生,深蓝色显色较少,心肌纤维走形平整。Western blot法结果显示,与IHF组比较,TSA组显著降低了由缺血性心力衰竭所造成的Galectin-3的上升(0. 52±0. 09 vs 1. 43±0. 18,P <0. 01)。结论丹参酮ⅡA可有效抑制缺血性心力衰竭大鼠心肌纤维化,其作用可能与抑制Galectin-3表达相关。

Objective To research the effect of tanshinone ⅡA on myocardial fibrosis in rats with ischemic heart failure, and to investigate its correlation with the expression of Galectin-3. Methods A total of Wistar rats were randomly divided into 3 groups： sham operation group （Sham）, ischemic heart failure group （IHF）, tanshinone ⅡA group （TSA）. To Establish an ischemic heart failure model, Tanshinone ⅡA group was given TSA pretreatment 1 hours before operation, the dose was 40mg/kg. Heart function was observed by echocardiography at 4 weeks after operation.The degree of myocardial fibrosis in each group was observed by Masson staining, and the expression of Galectin-3 protein was detected by Western blot. Results TSA group compared with IHF group, LVW/BW, HW/BW, LVW, HW, BW were significantly improved （ P 〈0.01）. Masson staining showed that TSA group significantly reduced the large collagen fiber hyperplasia caused by ischemic heart failure, and myocardial fibers were smooth. The results of Westernblot showed that compared with the IHF group, the TSA group significantly decreased the expression of Galectin-3 caused by ischemic heart failure （0.52±0.09 vs, 1.43±0.18, P 〈0.01）. Conclusion Tanshinone ⅡA effectively inhibited myocardial fibrosis in rats with ischemic heart failure, and its effect may be related to the inhibition of the expression of Galectin-3.