类风湿性关节炎患者外周血NKG2D、NKG2A及颗粒酶B表达的研究

Investigation on the Peripheral Blood Expressing NKG2D,NKG2A,Granzyme B of Patients with Rheumatoid Arthritis

目的本研究旨在探讨类风湿性关节炎(rheumatoid arthritis,RA)患者外周血中NK细胞表达NKG2D、NKG2A及胞内颗粒酶B的水平,并且分析它们与疾病活动度(DAS28)的相关性。方法流式细胞术检测41例RA患者和35例健康对照者外周血中NK细胞、NKG2D+NK细胞、NKG2A+NK细胞以及颗粒酶B+NK的表达水平;采用Spearman秩相关分析法分析它们与DAS28的相关性。结果 RA患者外周血中,NK细胞表达水平显著低于健康对照组(P=0. 016); NKG2D的表达水平显著低于健康对照组(P=0. 002),且NKG2D的表达水平与DAS28评分呈负相关(P <0. 05); NKG2A的表达水平与健康对照组之间比较差异无统计学意义(P=0. 547),且与DAS28评分不存在显著相关性(P> 0. 05);颗粒酶B的表达水平有所降低(P=0. 034),但与DAS28评分不存在显著相关性(P> 0. 05)。结论活化性受体NKG2D以及胞内颗粒酶B表达的异常降低可能导致NK细胞杀伤活性的损伤,不能有效杀伤免疫效应细胞,从而促进了RA的发生、发展。

Objective To investigate the expression of NKG2D,NKG2A, intracellular granzyme B in NK cells from patients with rheumatoid arthritis （RA） and their associations with the disease activity. Methods We studied 41 RA patients as well as 35 healthy controls. NK,NKG2D,NKG2A,granzyme B expression were analyzed by flow cytometry in peripheral blood mononuclear cells （PBMC）. Results We found that NK cells from RA patients as well as NKG2D and granzyme B showed a diminished expression compared with healthy controls（ P 〈0.05） ; but NKG2A had no significant difference between RA and healthy controls（ P =0.547）. Spearman analysis showed that the levels of NKG2D was negatively associated with DAS 28 score（ P 〈0.05）, but no significant correlation were detected between the levels of NKG2A and DAS 28 score as well as granzyme B （ P 〉0.05）. Conclusion The lower expression of activating receptor NKG2D and intracellular granzyme B may lead to the killing activity damage of NK cells, can not effectively killing immune effector cells, thus contributing to the development of RA.