肺炎支原体抗体检测的两种方法学比较

Comparison of two kinds of methodology for detecting mycoplasma pneumonia antibodies

目的比较化学发光免疫分析(CLIA)法与被动凝集(PA)法检测肺炎支原体(MP)抗体结果的一致性,评估两种方法对于MP感染的诊断价值。方法选择2017年6-10月在该院就诊的呼吸道感染患者268例,分别采用两种方法测定血清MP抗体,分析两种方法检测结果的一致性。结果 CLIA法与PA法检测MP抗体的总符合率为79.1%,一致性检验Kappa值为0.566(P<0.01);MP-IgM在婴幼儿和青少年中的阳性率较高,而MP-IgG在成人及老年人中的阳性率较高。结论 CLIA法与PA法检测MP抗体存在一致性,应用CLIA法同时检测MP-IgG和MP-IgM亚型可用于临床MP感染的诊断。

Objective To compare the consistency of the results between passive agglutination（PA）method and chemiluminescence immunoassay（CLIA）for detecting mycoplasma pneumonia（MP）antibodies,and to evaluate the diagnostic value of the two methods in MP infection.Methods Two hundred and sixty-eight patients with respiratory tract infection in the hospital from June to October 2017 were selected.Serum antibody levels were detected by using the two methods.Then the consistency of results detected by the two methods was analyzed.Results The total coincidence rate of PA and CLIA for detecting MP antibodies was79.1%,with the Kappa value of 0.566（P〈0.01）.The positive rate of MP-IgM was higher in infants and adolescents groups,while that of MP-IgG was higher in adults and elderly groups.Conclusion CLIA shows the consistency with PA for detecting MP antibodies.Using CLIA method for simultaneously detecting MP-IgG and IgM subtype can be used in the diagnosis of clinical MP infection.