两种方法检测HBV基因分型比较及基因型检出率与HBV DNA定量的关系

Comparison of two methods for detecting genotype and relationship between genotype detection rate and HBV DNA quantification

目的分析磁珠法与煮沸法检测乙型肝炎病毒(HBV)基因分型的优势,进一步分析基因型检出率与HBV DNA定量的关系。方法选取2013年10月至2016年7月、2017年1月至2018年1月该院感染病科就诊的1 943例慢性乙型肝炎患者作为研究对象。其中2013年10月至2016年7月采用提取HBV DNA,进一步采用荧光探针聚合酶链反应(PCR)检测乙型肝炎基因分型。2017年1月至2018年1月采用Abbot m2000提取的HBV DNA(磁珠法),进一步采用荧光探针PCR检测乙型肝炎基因分型。比较两种检测方法的结果差异。根据基因分型结果分析基因分型的检出率与HBV DNA定量水平的关系。结果磁珠法提取的HBV基因型检出率显著提高(P<0.05),但是磁珠法检测C型检出率降低,B、D型检出率更高(P<0.05)。HBV基因型检出率随着HBV DNA水平的升高而升高(P<0.05)。结论相对于煮沸法提取的HBV DNA,磁珠法更能够提高乙型肝炎基因分型阳性率,并且能够提高非C型以及低病毒载量的患者检出率,更值得应用于临床。

Objective To analyze the superiority of immunomagnetic beads method and boiling method for detecting hepatitis B virus（HBV）genotye and to further analyze the relationship between genotype detection rate and HBV DNA,quantification.Methods A total of 1 943 patients with chronic hepatitis B in the infectious department of this hospital from October 2013 to July 2016,January 2017 to January 2018 were selected as the research subjects.Among them,the boiling lysis method from October 2013 to July 2016 was adopted to extract HBV DNA,and further adopted fluorescence probe polymerase chain reaction（PCR）to detect hepatitis B genotype;the Abbot M2000（magnetic beads method）from January 2017 to January 2018 was adopted to extract HBV DNA,and further adopted fluorescence probe PCR to detect hepatitis B genotype.The differences between of results were compared between the two detection methods.The relationship between the genotype detection rate and HBV DNA quantitative level was analyzed according to genotyping results.Results The detection rate of magnetic bead method for extracting HBV genotype was significantly increased（P〈0.05）,but the detection rate of magnetic bead method for detecting genotype C was decreased and which for detecting genotype B and D was higher（P〈0.05）.The detection rate of HBV genotype was increased with the increase of HBV DNA level（P〈0.05）.Conclusion Compared with the boiling method for extracting HBV DNA,the magnetic beads method can more increase the positive rate of HBV genotyping,and can increase the detection rate of the patients with non-genotype C and low viral loading,which is more worthy of clinical application