卡瓦叶子的化学成分研究

Investigation on the Chemical Constituents from the Leaves of Kava

以卡瓦叶子粉末为研究对象,通过硅胶柱层析、薄层色谱法(TLC)跟踪、凝胶柱层析等方法对各个部位进行分离纯化,然后借助于核磁共振氢谱、碳谱、质谱等方法进行结构鉴定,最终从卡瓦叶子中分离出四个化合物,分别为:Dihydrokawain(1),5,7,4'-trimethoxyflavanone(2),芫花素(3), 5-hydroxy-7, 4'-dimethoxy-flavone(4)。采用MTT法,对四个化合物进行药理活性测试,结果发现,化合物1对细胞A549和细胞NCI-H460的IC50值分别为38.8136和38.5572μg/mL,有一定的细胞毒性,化合物2、3、4对细胞A549和NCI-H460没有明显的细胞毒性。

Based on the investigation on the Chemical Constituents from the leaves of kava, four compounds were obtained from the EtOAc extract by a combination of column chromatography of silica gel, Sephadex LH-20 mamy times. Their structures were determined on the basis of 1HNMR, 13C NMR, 2DNMR, UV, IR, and ESIMS spectradata and were identifiedas as Dihydrokawain （1）,5,7,4＇-trimethoxyflavanone （2）,genkwanin （3）, 5- hydroxy-7, 4＇-dimethoxy-flavone （4） The MTT assasy tested four compounds cytotoxicity： the result showed that compound 1 was found to be Moderate cytotoxicity against A549 and NCI-H460 Cells with IC50 values of 38.8136, 385572μg/mL respectively： Compound 2,3 and 4 were found to be inactive toward all the tested cell A549 and NCI-H460 （IC50〉50μgmL^-1）.