响应面法优化超声辅助离子液体提取银杏叶总黄酮

Optimization in Ionic Liquid Based Ultrasonic-Assisted Extraction of Total Flavonoids from Ginkgo biloba Leaves by Response Surface Methodology

以离子液体浓度、提取时间、提取温度、料液比为考察因素,以银杏叶总黄酮提取率为评价指标,采用响应面法优化超声辅助离子液体提取银杏叶总黄酮。结果表明,在料液比为1∶40(g∶mL)、提取时间为22min、离子液体浓度为1.1mol·L^(-1)、提取温度为54℃的最优条件下,银杏叶总黄酮提取率可达到26.48mg·g^(-1),与预测值(26.98mg·g^(-1))接近。该方法稳定可行,重复性好,可用于提取银杏叶总黄酮。

Using ionic liquid concentration,extraction time,extraction temperature,and solid-liquid ratio as influential factors,and the extraction rate of total flavonoids from Ginkgo biloba leaves as an evaluation index,we optimized the process of ionic liquid based ultrasonic-assisted extraction of total flavonoids from Ginkgo biloba leaves by response surface methodologies.The results show the extraction rate of total flavonoids reaches 26.48 mg·g ^-1 ,which is closed to the predictive value（26.98 mg·g^ -1 ） under the optimal conditions as follows：the solid-liquid ratio of 1∶40（g∶mL）,the extraction time of 22 min,the ionic liquid concentration of 1.1 mol·L ^-1 ,and the extraction temperature of 54 ℃.The method is stable and reproducible,and can be used to extract total flavonoids from Ginkgo biloba leaves.