甘蔗蔗糖磷酸合成酶基因SPS Ⅳ电子克隆及生物信息学分析

In silico Cloning and Bioinformatic Analysis of Sucrose Phosphate Synthase Gene SPS Ⅳ in Sugarcane

蔗糖在植物生长发育过程中发挥重要作用,蔗糖磷酸合成酶(SPS)是蔗糖合成的关键酶之一。本研究以通过克隆获得的甘蔗SPS Ⅳ基因部分DNA序列为探针进行电子克隆,获得甘蔗SPS Ⅳ的编码区序列全长,并采用生物信息学方法,对该基因编码蛋白的氨基酸组成、理化性质、亚细胞定位及高级结构等方面进行预测分析。结果表明:Sc SPS Ⅳ基因编码区全长2943 bp,编码981个氨基酸,Sc SPS IV可能是不稳定的亲水性非分泌蛋白,主要表达于细胞质。遗传进化分析表明,该基因与高粱、玉米蔗糖磷酸合成酶基因同源性高。以上研究结果为Sc SPS Ⅳ基因下一步进行分子克隆、功能验证和应用提供基础。

Sucrose plays a critical role in the process of plant growth and development. Sucrose phosphate synthase（SPS） is a key enzyme in sucrose biosynthesis pathway. In this study, the full length of sugarcane SPS Ⅳ coding region sequence was cloned in silico by using the partial sugarcane SPS Ⅳ DNA sequence as a probe. Some characters of the amino acid sequence encoded by Sc SPS Ⅳ gene, including composition of amino acid sequence, physical and chemical properties, subcellular localization, secondary and tertiary structure of protein, were analyzed by bioinformatics software. The results showed that the CDS sequence of Sc SPS Ⅳ gene was 2943 bp and encoding 981 amino acids, and Sc SPS Ⅳ might be an unstable hydrophilic non-secretory protein, which was mainly expressed in the cytoplasm. The phylogenetic analysis revealed that SPS genes from sugarcane, Sorghum and Zea mays were highly homologous. This study aims to provide the basis for further research of molecular cloning, functional analysis and applications.