2014至2017年广州无偿献血者梅毒检测分析

Determination and Analysis of Syphilis by ELISA and TPPA in Unpaid Blood Donors from 2014 to 2017

目的分析2014至2017年广州市无偿献血者梅毒检测的结果。方法采用酶联免疫吸附法(ELISA)进行梅毒血清学筛查,采用梅毒螺旋抗体明胶颗粒凝集试验检测法(TPPA)进行患者梅毒螺旋抗体的阳性确认。结果 2014至2017年广州市无偿献血者的梅毒检出率呈逐年升高趋势,差异具有统计学意义(P <0.05);不同性别、职业、年龄和不同文化程度的无偿献血者梅毒检出率差异具有统计学意义(P<0.05)。结论为了提高广州市采供血的血液安全,应对无偿献血者的血浆样本进行严格筛选筛查,减少和阻断梅毒的传播。

Objective To analyze the results of ELISA and TPPA in detecting syphilis in unpaid blood donation from 2014 to 2017 in Guangzhou. Methods Serological screening of syphilis was carried out by enzyme- linked immunosorbent assay （ELISA）, and positive results of syphilis antibody by TPPA were detected by using the syphilis spiral antibody gelatin particle agglutination test. Results The detection rate of syphilis in gratis blood donation from 2014 to 2017 in Guangzhou increased year by year with a statistically significant difference （P 〈 0.05 ）. There was a significant statistical difference in the detection rate of syphilis in unpaid blood donation patients with different gender, occupation, age and different educational levels, and the difference was statistically significant（P 〈0.05 ）. Conclusion In order to improve the blood safety of blood collection and supply in Guangzhou, the plasma samples from patients with unpaid blood donation should be screened to reduce and block the spread of syphilis.