乳酸脱氢酶联合免疫细胞化学检测在恶性胸腔积液诊断中的价值

The Clinical Value of Lactic Dehydrogenase Combined with Immunocytochemistry in the Diagnosis of Malignant Pleural Effusion

目的探讨乳酸脱氢酶(LDH)联合免疫细胞化学检测在恶性胸腔积液诊断中的价值。方法回顾性分析50例胸腔积液患者胸腔积液LDH检测浓度和细胞学标本分别做标记物癌胚抗原(CEA)、广谱细胞角蛋白(AE1/AE3)、钙视网膜蛋白(calretinin)、间皮细胞(mesothelial cell)、波形蛋白(vimentin)、细胞增殖指数(Ki-67)免疫细胞化学染色。结果癌症组LDH含量(850.60±126.58 U/L)显著高于对照组LDH含量(125.68±32.45 U/L),差异具有统计学意义(P <0. 01)。结论 LDH联合检测CEA、AE1/AE3、calretinin、MC、Vimentin、Ki-67的表达情况,有助于鉴别胸腔积液的良、恶性,还可以进一步确定恶性胸腔积液中恶性肿瘤细胞的类型,对患者治疗方案的选择及预后的判断具有重要的临床意义。

Objective To investigate the value of LDH （ Lactic dehydrogenase ） combined with immunocytochemistry in the diagnosis of malignant pleural effusion （MPEs）. Methods The retrospective analysis of the LDH concentration and cytology specimens with pleural effusionwhich were labeled as CEA, AE1/AE3, Calretinin,Mesothelial Cell, Vimentin, Ki67 immunocytochemical staining were conducted in 50 patients. Results The average content of LDH （850.60 ± 126.58U/L in cancer group was significantly higher than the average content of LDH （125.68 ± 2512.45U/L） in control group （P 〈 0.01 ）. Conclusion LDH combined the expressions of CEA, AE1 / AE3, Calretinin, Mesothelial Cell （MC）, Vimentin, and Ki-67, canbetter identify the benign and malignant pleural effusions, and can also facilitate thedetermination of the type of malignant tumor cells in malignant pleural effusions . It has important clinical significance of the decisions of treatment options and prognosis of the patient.