吐温-20对消除血清中总HCG检测假阳性的影响与分析

An Analysis of Tween-20 Application for the Elimination of the HCG Test False Positive in Serum

目的试验表面活性剂吐温-20对消除血清中总HCG检测假阳性的作用,进而观察其对酶结合物稳定性及试剂盒精密度的影响。方法用普通酶结合物稀释液(20%新生牛血清+0.02mol/L PBS)配制的酶结合物作对照组,实验组添加不同浓度的吐温-20,考察吐温-20对酶结合物活性的影响。选择合适的吐温-20浓度添加到普通酶结合物稀释液中配制酶结合物,与对照组对比检测定值血清,考察吐温-20消除血清中HCG检测假阳性的作用。在上述实验基础上,对比检测低(L)、高(H)质控品各20孔,以CV的变化考察吐温-20对试剂盒检测精密度的影响。37℃7天破坏性实验,考察吐温-20对酶结合物稳定性的影响。结果酶结合物中添加不同浓度吐温-20对其活性无显著性影响,0.2%吐温-20可消除血清中HCG检测的假阳性,同时对阳性血测值无影响。酶结合物中添加0.2%吐温-20对试剂盒检测的精密度、对酶结合物稳定性均无显著性影响,不影响试剂盒的分析性能。结论酶结合物中添加吐温-20可以有效消除血清中HCG检测的假阳性,可广泛用于生产中。

Objective We performed an experiment to investigate the effects of tween-20 on the elimination of the HCG test false positive in serum, and then evaluated the precision of the kit and the stability of the enzyme bonder. Methods We set diluent enzyme bonder solution as control, and tween-20s with different concentration as treatment, then observed the changes of invariable serum between the control and treatment groups. Based on the results, we choose both 20 low-quality products and high-quality products to make comparison, to further evaluate the effects of tween-20 on accuracy by CV. We also exposed the enzyme bonder to 37℃ for 7days, then compared the control group and treatment group results. Results We showed that tween-20s with different concentration had no significant impact on the activity of the enzyme bonder. 0.2% tween-20 could eliminate the HCG test false positive with no effect on the positive in serum, and also had no significant impact on kit accuracy and enzyme bonder stability. Conclusion Binding enzyme bonder with tween-20 can effectively eliminate the HCG test false positive in serum. This can be widely applied in production.