萝卜叶乙醇提取物不同极性部位对果蝇寿命的影响

Effects of Different Polarity Fractions of Radish Leaf Ethanol Extracts on Lifespan of Drosophila melanogaster

以萝卜叶为研究对象,经乙醇提取、不同极性有机溶剂依次萃取获得石油醚部位(Petroleum ether fraction,PERs)、氯仿部位(Chloroform,CRs)、乙酸乙酯部位(Ethyl acetate fraction,EARs)、水饱和正丁醇部位(Water saturated butanol,WBRs)和水部位(Aqueous fraction,AQRs),并考察各部位的总酚、总黄酮含量及总抗氧化活性。以果蝇为模式生物,研究萝卜叶乙醇提取物不同极性部位对其寿命及生殖活性的影响。结果表明,EARs的总酚((78.58±0.51) mg GAE/g dw)、总黄酮((244.40±1.00) mg RE/g dw)含量及抗氧化活性最高。不同极性部位均能延长果蝇的寿命,并提高其生殖活性,其中以EARs效果最佳。经饲喂高剂量(15 mg/kg) EARs后,果蝇的平均寿命延长22.98%、最高寿命延长27.27%、交配持续期延长39.75%、交配潜伏期缩短72.12%。各不同极性部位均能提高果蝇SOD活性和MDA含量,且EARs和WBRs均能提高果蝇CAT活性。以上结果表明,萝卜叶乙醇提取物的EARs和WBRs具有延长果蝇寿命的作用。

Different polarity fractions of radish leaf ethanol extracts were obtained by petroleum ether,chloroform,ethyl acetate,n-butanol,and water,which named as PERs,CRs,EARs,WBRs,and AQRs. The total phenolic,flavonoid contents and total antioxidant activity of the five fractions were investigated. The anti-aging effects of different polarity fractions on Drosophila melanogaster were evaluated. Results showed that： EARs exhibited the highest total phenolic contents（（ 78.58 ± 0.51） mg GAE/g dw）,total flavonoid contents（（ 244.40 ± 1.00） mg RE/g dw）,and total antioxidant activity,followed by WBRs,CRs,PERs,and AQRs. All of the five fractions prolonged the lifespan in Drosophila melanogaster and the average lifespan,maximal lifespan,mating latency,mating duration were extended by 22.98%,27.27%,39.75%,and 72.12%,respectively,after treatment with EARs（ 15 mg/kg）. In addition,all of the five fractions enhanced the SOD activity and decreased the MDA level,but only EARs and WBRs decreased the CAT activity in Drosophila melanogaster. The results showed that EARs and WBRs of radish leaf ethanol extracts had effects of prolonging Drosphlia melangaster＇s lifespan.