微流控静态液滴阵列芯片制备非球形微粒

Non-Spherical Particles Prepared by Microfluidic Static Drop Array Chips

针对静态液滴阵列（SDA）微流控芯片中的毛细阀现象进行机制研究,探讨了流阻及表面张力对SDA芯片中微液滴的生成与存储过程的影响。通过Fluent软件模拟流体流动情况,并对照实验结果进行讨论,同时通过OpenCV图像处理技术实时监测流量变化,进一步验证毛细阀现象。提出了一种利用微结构模版法制备非球形微粒的新方法,通过将微液滴置于4℃低温环境下冷却固化,制得与芯片捕获结构相近的微米级非球形琼脂糖微粒,证实了该方法的可行性。该方法具有成本低、易操作、可控性高等优点,为未来制作非球形微粒提供了一个新途径。

The mechanism of capillary valve phenomenon in static droplet array（SDA）microfluidic chips was studied,and the influences of flow resistance and surface tension on the generation and storage processes of micro-droplets in SDA chips were discussed.The flow process was simulated by Fluent software,and the experimental results were discussed.Meanwhile,the flow change was monitored in real time by OpenCV image processing technology to further verify the capillary valve phenomenon.A new method for preparing non-spherical particles by using a micro-structured template method was proposed.The micro-droplets were cooled and solidified at 4℃,and then the nono-spherical agarose particles similar to the chip-capture structure were prepared,verifying the feasibility of the method.The method has advantages of low cost,easy operation,high controllability and provides a new way for preparing the micro-sized non-spherical particles in the future.