白首乌提取物C_(21)甾体苷对结直肠癌细胞株SW480的影响

Effects of C_(21) Steroidal Glycoside Extract from Radix Cynanchi Auriculati on Cell Proliferation,Invasion and Migration of Colorectal Cancer Cell Line SW480

目的:探讨白首乌提取物C_(21)甾体苷对人结直肠癌细胞株SW480生长、凋亡、侵袭和迁移的影响。方法:体外常规培养人结直肠癌细胞株SW480,以不同浓度的C_(21)甾体苷作用于细胞,分别培养24 h、48 h、72 h,采用溴化二甲噻唑二苯四氮法检测不同浓度的C_(21)甾体苷对人结直肠癌细胞株SW480生长的抑制作用;Annexin V-FITC/PI双染流式细胞仪检测细胞凋亡情况;Transwell小室法检测细胞的侵袭和迁移能力。结果:C_(21)甾体苷以时间和浓度依赖性抑制人结直肠癌细胞株SW480的生长,促进人结直肠癌细胞株SW480凋亡,抑制人结直肠癌细胞株SW480的侵袭和迁移。结论:C_(21)甾体苷可抑制人结直肠癌细胞株SW480的生长,该抑制作用与诱导其凋亡及降低其侵袭和迁移能力有关。

Objective ：To investigate the effects of Cn steroidal glycoside extract from Radix cynanchi auriculati on cell prolifera- tion,apoptosis,invasive and migration on human colon cancer cell SW480. Methods：Human colon cancer cell SW480 cells were cultured in vitro, and dealt by C21 sterols with different concentrations. MTY was performed to observe the proliferation at 24,48,72 h respectively. Annexin V-FITC/PI flow cytometry was used to determine the apoptosis rate. The invasion and migration of cells were detected by Transwell method. Results ： C21 sterols could inhibit the proliferation of SW480 cells in vitro, with manner of con- centration and time dependency. C21 steroidal glycoside extract could elevate the apoptosis rates, and inhibited the migration and invasive ability of human colon cancer cell SW480 ceils significantly. Conclusion ： C21 steroidal glycoside extract could inhibit the proliferation of human colon cancer cell SW480 cells, the mechanism of which may be related to the induction of cell apoptosis and the inhibition of cell invasion and migration.