苦蘵育种F_1群体真伪鉴定

Rapidly Identifying Hybrid F_1 Physalis angulata Using SSR

目的:研究苦蘵育种F_1群体真伪。方法:以浙江临海和浦江两个种源的苦蘵为亲本,对获得的85个杂交F_1代,利用200对全基因组SSR标记进行杂交后代真伪鉴定。结果:筛选出2对可进行苦蘵F_1代个体杂种鉴定的特异性SSR标记SSR0959和SSR0116。其中利用这两对SSR标记证实本研究构建的苦蘵杂交F_1代群体的85个单株均为双亲互补型(真杂交种)。结论:通过SSR分子标记技术可以快速、准确的鉴定出苦蘵杂交群体中的真杂交种,为后续苦蘵高密度遗传连锁图谱的构建以及有效成分的QTL定位研究奠定基础。

Objective：To evaluate the authenticity of F1hybrid Physalis angulate.Methods：Two P.angulate germplasm sources from Linhai and Pujiang of Zhejiang province were used as parents to make hybrid combinations and got 85 F1offspring for hybrid identification,with 200 SSR markers form whole genome wide.Results：For the 85 F1hybrids in this study,2 pairs of primers were selected as markers of hybrid identification.The true hybrid rate of 85 offsprings was 100%with both the male and female parent specific bands.Conclusion：The authenticity of F1hybrids could be easily validated by SSR markers,if whose parents had not visible and distinguishable phenotypic differences.The SSR-PCR rapid detection system for P.angulate hybrid identification might be great potential in the high density genetic map construction and effective constituents QTL mapping in future.