基于公共数据库和cDNA芯片研究CCT6A在肝细胞癌中的表达及意义

Expression of CCT6A and its prognostic role in hepatocellular carcinoma: Analysis based on the data-mining of ONCOMINE and cDNA array

目的分析CCT6A在肝癌中的表达及其与肝癌预后的关系。方法采用Oncomine、GEPIA、HPA数据库和肝癌cDNA芯片检测研究CCT6A在肝癌等恶性肿瘤及其癌旁组织中的差异表达,并且分析CCT6A在肝癌组织中的表达与肝癌预后的关系。结果 Oncomine数据库分析发现,CCT6A在乳腺癌、肺癌、胃癌、肝癌等多种癌组织中的表达显著高于其对应的正常组织。Oncomine、GEPIA、HPA数据库和肝癌cDNA芯片结果分析证实,CCT6A的mRNA和蛋白表达在肝癌组织均明显高于癌旁组织(P<0.05)。GEPIA数据库生存期分析,CCT6A在肝癌组织中高表达与肝癌患者生存期短密切相关。结论 CCT6A在肝癌组织中显著升高,且其高表达与肝癌患者的预后差密切相关。

Objective To explore the expression of CCT6A in hepatocellular carcinoma （HCC） patients, and analyze the correlation between CCT6A expression and prognosis of HCC patients. Methods Using the databases including Oncomine, GEPIA and HPA database, as well as cDNA microarray of HCC performing by QPCR analysis, CCT6A expression in HCC and the correlation between CCT6A expression and survival of HCC patients was analyzed. Results Base on Oncomine analysis, CCT6A expression in breast cancer, lung cancer, gastric cancer, liver cancer and other cancers were significantly increased comparing with their corresponding normal tissues. The analyzes from Oncomine, GEPIA, HPA databases and cDNA microarrayindicated that the expression of CCT6A in HCC tissues was significantly increased in HCC tissues comparing with corresponding normal tissues on both mRNA and protein levels. Survival analysis through GEPIA database indicated that high expression of CCT6A in HCC was significantly associated with shorter survival of HCC patients. Conclusion CCT6A is highly expressed in hepatocellular carcinoma, and increase of its expression is significantly associated with poor prognosis of HCC patients.