喜树碱通过抑制HSV-1复制相关基因的表达发挥抗HSV-1作用

Anti HSV-1 effect of camptothecin by inhibiting HSV-1 replication genes

目的探讨喜树碱体外抗单纯疱疹病毒Ⅰ型(HSV-1)的作用及其机制。方法不同浓度的喜树碱作用于HSV-1感染的Vero细胞后,采用细胞病变(cytopathologic effects,CPE)法评价喜树碱体外抗HSV-1的作用,计算喜树碱半数毒性浓度(TC_(50))、病毒半数抑制浓度(IC50)及治疗指数(TI),并通过实时荧光定量PCR(RT-qPCR)法检测喜树碱对HSV-1相关基因m RNA表达的影响。结果喜树碱的TC_(50)为2 153.44 nmol/L,IC_(50)为43.01 nmol/L,TI为50.07。RT-qPCR结果显示,喜树碱能明显抑制HSV-1 UL12、UL42、UL54、TK基因的表达,其抑制作用与喜树碱浓度呈剂量依赖关系。结论喜树碱具有明显抗HSV-1的作用,能抑制HSV-1 UL12、UL42、UL54、TK基因的转录。

Objective To study the antiviral effect and mechanism of camptothecin against HSV-1 in vitro. Methods Adding different concentration of camptothecin to the Vero cells infected by HSV-1, the measure of cytopathologic effects（CPE） was taken to evaluate the result of antiviral effect and mechanism of camptothecin. The median toxic concentration（TC50）, median inhibitory concentration（IC50）, and therapeutic index（TI） of camptothecin were calculated, and the affection of camptothecin on HSV-1 m RNA expression was examined through the method of RT-q PCR. Results TC50 of camptothecin was 2 153.44 nmol/L, IC50 was 43.01 nmol/L, and TI was 50.07. The results of RT-q PCR showed that camptothecin can significantly inhibit the gene transcription of HSV-1, UL12, UL42, UL54, and TK, and its inhibitory effect was in dose-dependence relationship with the concentration of camptothecin. Conclusion Camptothecin has an obvious antiviral effect on HSV-1 and can inhibit the transcription of HSV-1 genes such as UL12, UL42, UL54, and TK.