穴位埋线对非酒精性脂肪肝模型大鼠脂质代谢及AdipoR2-PPARα-CPT1a信号通路mRNA表达水平的影响

Effect of acupoint catgut embedding on lipid metabolism and mRNA expression of adipoR2-PPARα-CPT1a signaling pathway of nonalcoholic fatty liver model rats

试验选用48只雌性Wistar大鼠,随机分为对照组和模型组。高脂饲料饲喂8周诱导非酒精性脂肪肝模型。模型验证后,将大鼠分为空白对照组、空白埋线A组、空白埋线B组、模型组、模型埋线A组、模型埋线B组,每组6只。埋线组进行穴位埋线,A组：肝俞、胆俞、后海;B组：肝俞、胆俞、后海、后三里。2周后检测血清TG、TC、FFA、LDL-C、HDL-C、ADPN含量及肝脏TG、TC、FFA、ACC、FAS含量;RT-PCR检测肝脏AdipoR2、PPARα、CPT-1a mRNA表达水平。结果显示：与模型组相比,模型埋线A、B组血清HDL-C、ADPN含量显著升高,TC/HDL-C、TG/HDL-C比值显著降低;模型埋线A、B组肝脏TG含量显著降低;模型埋线B组肝脏FFA、FAS含量显著降低;模型埋线A、B组肝脏PPARαmRNA表达水平显著升高（P〈0.05）。结果表明：穴位埋线对非酒精性脂肪肝模型大鼠血脂指标有一定的改善作用,能有效降低肝脏TG、FAS含量,调节肝脏脂质的合成,其机理可能与其参与PPAR通路调节有关。

Forty-eight female Wistar rats were randomly divided into control group and model group. The model group rats were fed the high-fat diet for 8 weeks to induce non-alcoholic fatty liver. After model was validated, rats were divided into control group, control ＋ embedding A group, control＋ embedding B group, model group, model＋ embedding A group, model＋ embedding B group,6 per group. Collagen suture were implanted into embedding groups,Ganshu＋Danshu＋ Houhai in A group and Ganshu＋Danshu＋Houhai＋Housanli in B group. Two weeks later,serum levels of TG, TC, FFA, HDL-C, LDL-C and ADPN were measured. Furthermore, changes and comparisons of liver TG,TC,FFA,ACC and FAS content were observed. The mRNA expression levels of AdipoR2 ,PPARα and CPT-la were investigated by RT-PCR. Rats in model＋embedding A,B group were found to have significantly higher level in serum HDL-C and ADPN and less level in serum TG/HDL-C and TC/HDL-C than those in the model group（P〈0.05）. Model＋embedding A,B group was effective on reducing liver TG and model＋embedding B group decreased liver FAS obviously. Compared with model group, mRNA expressions of PPARα increased in model＋ embedding A,B group（P〈0.05）. Acupoint catgut embedding improved the serum lipid conditionof nonalcoholic fatty liver model rats, reducing TG and FAS content in liver, which associated with the regulation of lipid synthesis. The mechanism may be related to its involvement in PPAR pathway regulation.