高钒对雏鸡肾脏生长及结构、Na^+-K^+-ATP酶活性和血清肌酐、尿酸含量的影响

Effect of high vanadium on the renal growth and structure,the activities of renal ATPase and the content of serum creatinine and uric acid in chicken

选用1日龄AA肉鸡健雏420只,随机分为6组,每组70只,分别饲以对照日粮（钒0.073mg/kg）和高钒日粮（钒5mg/kg,高钒Ⅰ组;钒15mg/kg,高钒Ⅱ组;钒30mg/kg,高钒Ⅲ组;钒45mg/kg,高钒Ⅳ组;钒60mg/kg,高钒Ⅴ组）42d,以实验病理学和生化试剂盒检测的方法观察研究雏鸡肾脏变化。结果显示：高钒Ⅱ、Ⅲ、Ⅳ、Ⅴ组肾脏质量显著降低（P〈0.05）,同时肾小管上皮细胞发生不同程度颗粒变性和空泡变性;高钒Ⅳ、Ⅴ组Na^＋-K^＋-ATP酶活性显著降低（P〈0.01）,高钒Ⅳ、Ⅴ组血清肌酐、尿酸含量显著增加（P〈0.05）。结果表明：高钒含量超过15mg/kg时对雏鸡肾脏可造成一定损伤,引起肾脏功能下降。

The objective of this study was to clarify the lesion of kidney by the effects of high vana- dium on the kidney growth and structure,the activities of renal Na＋-K＋-ATPase and the contents of serum creatinine and uric acid in broilers. Four hundred and twenty one-day-old broilers were divided into six groups and fed on a corn-soybean basal diet as control diet （vanadium 0. 073 mg/kg） or the same diet amended to contain 5,15,30,45 and 60 mg/kg vanadium （high vanodium groupsⅠ , Ⅱ , Ⅲ , Ⅳ, Ⅴ ） supplied as ammonium metavanadate for 42 days. As tested by the methods of experimental pathology and biochemical reagent kits, the kidney weight were significantly lower （P〈0.05） in high vanadium groups Ⅱ , Ⅲ, Ⅳ and V than in control group and there was a certain granular and vacuolar degeneration in renal tubular epithelial cells in high vanadium groups IV and V. Meanwhile, the Na＋-K＋-ATPase activities of kidney were significantly lower （P〈0. 01） in high vanadium groups IV and V than in control group while the serum creatinine and uric acid contents were significantly increased（P〈0.05）. As the results showed high vanadium over 30 mg/kg could induce lesion in kidney in chicken.