ApoE和IL-18基因多态性与脑胶质瘤发生的临床关系

The Clinical Relationship between ApoE and IL-18 Gene Polymorphism and Brain Glioma

为了探讨载脂蛋白E （ApoE）、白介素18 （IL-18）基因多态性与脑胶质瘤发生的临床关系,本研究2013年8月至2016年9月收治的194例脑胶质瘤患者作为病例组,选择同时期本院体检中心的194例健康人员作为对照组。采用问卷调查法收集两组人员基本资料;采用酶联免疫吸附实验（ELISA）检测血清ApoE和IL-18水平;采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析ApoE基因多态性;采用序列特异引物聚合酶链反应（SSP-PCR）法检测IL-18基因启动子区-137G/C突变位点多态性。对比脑胶质瘤患者与健康人群血清中ApoE和IL-18含量,ApoE和IL-18各基因型和等位基因频率的分布情况。研究显示,病例组血清ApoE水平明显高于对照组（p〈0.05）,病例组血清IL-18水平明显低于对照组（p〈0.05）。病例组ApoE和IL-18基因型及等位基因频率与对照组相比均差异显著（p〈0.05）。研究表明,脑胶质瘤患者血清ApoE和IL-18水平异常,ApoE和IL-18各基因型和等位基因频率明显改变,提示ApoE和IL-18基因多态性与脑胶质瘤的发生有密切相关。

In ordet to investigate the clinical relationship between ApoE and IL-18 gene polymorphism and brain glioma. 194 brain glioma patients treated in our hospital from August 2013 to September 2016 were selected as case group, and 194 healthy people in medical center of our hospital were selected as control group during the same period. The basic data of the two groups were collected by questionnaire survey. Serum ApoE and IL-18 levels were detected by enzyme-linked immunosorbent assay （ELISA）. Polymerase Chain Resction-restriction fragment length polymorphism （PCR-RFLP） was used to analyze ApoE gene polymorphism. The sequence specific primers polymerase chain reaction （SSP-PCR） was used to detect the polymorphism oflL-18 （-137G/C）. The distribution of ApoE and IL-18 in serum and the frequencies of ApoE and IL-18 genotypes and alleles in glioma patients and healthy people were compared. Results showed that, the serum ApoE level in the case group was significantly higher than that in the control group （p 〈0.05）, and the serum IL-18 level was significantly lower than the control group （p〈0.05）. The frequency of ApoE and IL-18 genotypes and alleles of case group were significantly different from those in the control group （p〈0.05）. Studies had shown that glioma patients with abnormal levels of serum ApoE and IL- 18, ApoE and IL- 18 genotype and allele frequency significantly changed, suggesting that ApoE andIL-18 gene polymorphisms are closely related to the occurrence of glioma.