安岳地区女性高危型HPV基因分型结果分析

Analysis of the Results of High-risk HPV Genotyping in Women in Anyue Area

目的分析安岳地区女性高危型人乳头瘤病毒的感染以及各亚型的分布情况,为本地区宫颈癌防治及流行病学研究提供依据。方法收集2016年1月～2017年12月年安岳地区自愿进行15种高危型HPV检测的女性宫颈脱落细胞标本2078例,应用实时荧光定量PCR进行包括16、18、31、33、35、39、45、51、52、53、56、58、59、66、68在内的15种HR-HPV检测,分析HRHPV在人群中的总体和不同年龄段感染情况以及各亚型的分布特点。结果 2078例女性宫颈脱落细胞标本总阳性检出率为22.81%,其中单一感染占17.37%,混合感染占5.44%;在各年龄组中<20岁组与>60岁年龄组HR-HPV感染率最高,差异具有统计学意义(P<0.05);15种高危型中检出前五位的依次为:52型、16型、31型、53型、58型。结论本地区女性人群的高危型HPV检出率较高,对本地区相关人群进行高危型HPV筛查非常必要。

Objective To analyze the infection and distribution of various subtypes of high-risk human papillomavirus in women inAnyue area, and to provide evidence for prevention and epidemiological studies of cervical cancer in this area.Methods A total of2078 specimens of cervical exfoliated cells from 15 high-risk HPV patients in the Anyang area from January 2016 to December 2017were collected and analyzed by real-time fluorescent quantitative PCR 15 HR-HPV tests including 16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,analyzing the overall and HR-HPV in the population infection at different ages and distribution characteristics of eachsubtype.Results The total positive rate of cervical cancer cells exfoliated in 2078 cases was 22.81%,of which single infectionaccounted for 17.37%,mixed infection accounted for 5.44%;HR-HPV infection rate in〈20 years old group and〉60 years old age groupin all age groups highest,the difference was statistically significant（ P〈0.05）;the top five of the 15 high-risk types were：52,16,31,53 and58.Conclusion The high-risk HPV detection rate of female population in this region is high,which is necessary for high-risk HPVscreening in relevant populations in this region.