普通野生稻根特异启动子的克隆与鉴定

Cloning and Identification of a Promoter with Root Tissue-specific Expression in Common Wild Rice

通过普通野生稻转录组文库筛选到一个普通野生稻(Oryza rufipogon Griff.)根特异表达基因,并克隆启动子序列,命名为Or RSGp,长度为896 bp,含有CAAT-box、TATA-box等主要功能元件,还有参与茉莉酸甲酯反应的响应元件,以及抗旱、抗逆等响应元件。将其与gus报告基因融合转化拟南芥,GUS组织化学染色与定量分析结果表明,Or RSGp调控gus基因在根部特异表达。

Based on previous transcriptome data, a root-specific expression gene of common wild rice （ Oryza rufipogon Griff. ） was screened, and the promoter sequence was cloned and designated as OrRSGp. It was 896 bp in length and contained main functional elements such as CAAT-box and TATA-box, MeJA-responsiveness and some elements involved in defense and stress responsiveness. It was fused with the β-glucuronidas （ gus ） reporter gene and transformed into Arabidopsis thaliana. GUS histochemical staining and quantitative analysis showed that OrRSGp regulated gene gus specific expression in roots.