大鼠宫颈上皮细胞的原代培养

Primary culture of rat cervical epithelial cells

目的探索大鼠宫颈上皮细胞的培养方法。方法分离大鼠宫颈上皮细胞,采用上皮细胞培养无血清培养基,添加牛脑提取物和表皮生长因子(EGF)进行培养。结果大鼠宫颈上皮细胞培养8 d后,可以在显微镜下观察到宫颈上皮细胞从组织碎片中移出,大片贴在培养瓶壁上,成集落状生长。结论使用上皮细胞无血清培养基添加EGF、牛脑提取物可以实现大鼠宫颈上皮细胞的原代培养。

【Objective】To introduce a new method to culture rat cervical epithelial cells.【Methods】Cervical epithelial cells separated from rats were cultured in serum-free medium that contains epidermal growth factor（EGF） and bovine pituitary extract. 【Results】After 8 days in culture, we can see cervical epithelial cells were removed from the tissue fragments and attached to the culture bottle wall to grow into colonies of cells, through microscope.【Conclusion】Rat cervical epithelial cells can be cultured primarily in serum-free medium that contains EGF and bovine pituitary extract.